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Invitrogen
IL36B Polyclonal Antibody
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FIGURE: 1 / 2
IL36B Antibody (PA5-100833) in ICC/IF
Immunocytochemistry analysis of IL36B in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with IL36B polyclonal antibody (Product # PA5-100833) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100833
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human IL36B(Accession Q9NZH7), corresponding to amino acid residues W126-M164.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total IL1F8.
Target Information
IL-36B is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL-36B is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP. Like the related proteins IL-36A and IL-36G, IL-36B requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown. The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; cytokine; hIL-1H2; family of interleukin 1-eta; FIL1 eta; IL-1 eta; IL-1F8; IL-1F8 (FIL1-eta); Interleukin; interleukin 1 family, member 8; interleukin 1 family, member 8 (eta); interleukin-1 eta; interleukin-1 family member 8; interleukin-1 homolog 2; Interleukin-1 Superfamily e; similar to IL-1; unnamed protein product
Gene Aliases: 2310043N20Rik; FIL1; FIL1-(ETA); FIL1H; FILI-(ETA); If36b; IL-1F8; IL-1H2; IL1-ETA; IL1F8; IL1H2
UniProt ID: (Human) Q9NZH7, (Mouse) Q9D6Z6
Entrez Gene ID: (Human) 27177, (Mouse) 69677
inflammatory response
immune response
cytokine-mediated signaling pathway
positive regulation of T cell differentiation
cellular response to lipopolysaccharide
positive regulation of cytokine production involved in inflammatory response
positive regulation of cytokine production
positive regulation of interleukin-6 production
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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