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Invitrogen
PLCE1 Polyclonal Antibody
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FIGURE: 1 / 14
PLCE1 Antibody (PA5-100856) in ICC/IF
Immunofluorescent analysis of PLCE1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and PLCE1 polyclonal antibody (Product # PA5-100856) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100856
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PLCE1(Accession Q9P212), corresponding to amino acid residues R2267-Q2302.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PLCE1.
Target Information
This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pancreas-enriched phospholipase C; phosphoinositide phospholipase C; phosphoinositide phospholipase C-epsilon-1; phosphoinositide-specific phospholipase C epsilon-1; phospholipase C epsilon; PLC-epsilon-1; pPLC; similar to CePLC210; unnamed protein product
Gene Aliases: 4933403A21Rik; mKIAA1516; NPHS3; PLCE; PLCepsilon; PPLC
UniProt ID: (Human) Q9P212, (Mouse) Q8K4S1, (Rat) Q99P84
Entrez Gene ID: (Human) 51196, (Mouse) 74055, (Rat) 114633
lipid metabolic process
diacylglycerol biosynthetic process
signal transduction
epidermal growth factor receptor signaling pathway
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
small GTPase mediated signal transduction
Ras protein signal transduction
positive regulation of lamellipodium assembly
glomerulus development
intracellular signal transduction
phosphatidylinositol metabolic process
phosphatidylinositol-mediated signaling
release of sequestered calcium ion into cytosol
calcium-mediated signaling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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