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Invitrogen
TRIO Polyclonal Antibody
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FIGURE: 1 / 4
TRIO Antibody (PA5-100920) in ICC/IF
Immunofluorescent analysis of TRIO in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TRIO polyclonal antibody (Product # PA5-100920) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100920
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TRIO(Accession O75962), corresponding to amino acid residues K667-Q717.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TRIO.
Target Information
TRIO promotes the exchange of GDP by GTP. Together with leukocyte antigen-related (LAR) protein, it could play a role in coordinating cell-matrix and cytoskeletal rearrangements necessary for cell migration and cell growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PTPRF-interacting protein; triple functional domain (PTPRF interacting); unnamed protein product
Gene Aliases: 6720464I07Rik; ARHGEF23; MEBAS; MRD44; MRD63; Solo; tgat
UniProt ID: (Human) O75962, (Rat) F1M0Z1, (Mouse) Q0KL02
Entrez Gene ID: (Human) 7204, (Rat) 310192, (Mouse) 223435
axon guidance
negative regulation of fat cell differentiation
neuron projection morphogenesis
modulation of synaptic transmission
postsynaptic modulation of chemical synaptic transmission
transmembrane receptor protein tyrosine phosphatase signaling pathway
regulation of small GTPase mediated signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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