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Invitrogen
HFE Polyclonal Antibody
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FIGURE: 1 / 5
HFE Antibody (PA5-102408) in ICC/IF
Immunofluorescent analysis of HFE in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and HFE polyclonal antibody (Product # PA5-102408) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-102408
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HFE(Accession Q30201), corresponding to amino acid residues Q119-W169.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total HFE.
Target Information
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin. It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hemochromatosis; hereditary hemochromatosis protein HLA-H; HFE 1; high Fe; HLA H; MGC10379; MGC103790; MGC:150812; MHC class I-like protein HFE; RT1-CAFE; unnamed protein product
Gene Aliases: HFE1; HH; HLA-H; MR2; MVCD7; TFQTL2
UniProt ID: (Human) Q30201, (Rat) O35799, (Mouse) P70387
Entrez Gene ID: (Human) 3077, (Rat) 29199, (Mouse) 15216
antigen processing and presentation of peptide antigen via MHC class I
regulation of T cell mediated immunity
negative regulation of T cell cytokine production
cellular iron ion homeostasis
receptor-mediated endocytosis
cell surface receptor signaling pathway
response to iron ion
positive regulation of gene expression
antigen processing and presentation
BMP signaling pathway
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
transferrin transport
regulation of iron ion transport
positive regulation of receptor-mediated endocytosis
positive regulation of SMAD protein import into nucleus
multicellular organismal iron ion homeostasis
protein-containing complex assembly
cellular response to iron ion
positive regulation of peptide hormone secretion
negative regulation of antigen processing and presentation of endogenous peptide antigen via MHC class I
response to iron ion starvation
regulation of protein localization to cell surface
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of CD8-positive, alpha-beta T cell activation
acute-phase response
female pregnancy
cellular response to iron ion starvation
gene expression
hormone biosynthetic process
urate metabolic process
regulation of catalytic activity
liver regeneration
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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