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Invitrogen
Glypican 3 Polyclonal Antibody
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FIGURE: 1 / 6
Glypican 3 Antibody (PA5-102528) in ICC/IF
Immunofluorescent analysis of Glypican 3 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Glypican 3 polyclonal antibody (Product # PA5-102528) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 4... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-102528
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GPC3(Accession P51654), corresponding to amino acid residues R39-M89.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total GPC3.
Target Information
GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell surface protein; precursor core protein cleaved by furin; defective in Simpson-Golabi-Behmel overgrowth syndrome; glypican proteoglycan 3; GTR2-2; GTR22; heparan sulphate proteoglycan; intestinal protein OCI-5; OCI-5; proteoglycan GPC3; secreted glypican-3; unnamed protein product
Gene Aliases: DGSX; GTR2-2; MXR7; OCI-5; SDYS; SGB; SGBS; SGBS1
UniProt ID: (Human) P51654, (Mouse) Q8CFZ4, (Rat) P13265
Entrez Gene ID: (Human) 2719, (Mouse) 14734, (Rat) 25236
anatomical structure morphogenesis
regulation of signal transduction
cell migration
cell migration involved in gastrulation
positive regulation of protein catabolic process
positive regulation of endocytosis
negative regulation of smoothened signaling pathway
regulation of canonical Wnt signaling pathway
coronary vasculature development
cell proliferation involved in kidney development
mesenchymal cell proliferation involved in ureteric bud development
mesonephric duct morphogenesis
positive regulation of canonical Wnt signaling pathway
regulation of protein localization to membrane
regulation of non-canonical Wnt signaling pathway
branching involved in ureteric bud morphogenesis
kidney development
negative regulation of cell proliferation
response to bacterium
organ morphogenesis
anterior/posterior axis specification
body morphogenesis
bone mineralization
osteoclast differentiation
lung development
positive regulation of BMP signaling pathway
embryonic hindlimb morphogenesis
regulation of growth
positive regulation of smoothened signaling pathway
negative regulation of growth
positive regulation of glucose import
negative regulation of epithelial cell proliferation
cell proliferation involved in metanephros development
negative regulation of canonical Wnt signaling pathway
positive regulation of Wnt signaling pathway, planar cell polarity pathway
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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