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Invitrogen
GTF2A1 Polyclonal Antibody
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FIGURE: 1 / 3
GTF2A1 Antibody (PA5-102739) in ICC/IF
Immunofluorescent analysis of GTF2A1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and GTF2A1 polyclonal antibody (Product # PA5-102739) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-102739
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GTF2A1(Accession P52655), corresponding to amino acid residues D284-V334.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total GTF2A1.
Target Information
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A) and several general initiation factors. One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: general transcription factor 2a, 1; general transcription factor IIA 1; general transcription factor IIA subunit 1; general transcription factor IIa, 1 (37kD and 19kD subunits); general transcription factor IIA, 1, 19/37kDa; glucose regulated protein, 58kD pseudogene; TFIIA alpha/beta; TFIIA alpha/beta subunits; TFIIA large subunit; transcription initiation factor TFIIA 42 kDa subunit; unnamed protein product
Gene Aliases: 6330549H03Rik; Tf2a; TF2A1; TFIIA; TFIIA-42; Tfiia1; TfIIAa/b; TFIIAL
UniProt ID: (Human) P52655, (Rat) O08949, (Mouse) Q99PM3
Entrez Gene ID: (Human) 2957, (Rat) 83830, (Mouse) 83602
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II general transcription initiation factor binding
DNA binding
protein binding
obsolete general RNA polymerase II transcription factor activity
TBP-class protein binding
protein heterodimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription initiation from RNA polymerase II promoter
biological_process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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