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Invitrogen
RAD21 Polyclonal Antibody
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FIGURE: 1 / 5
RAD21 Antibody (PA5-102839) in ICC/IF
Immunocytochemistry analysis of RAD21 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RAD21 polyclonal antibody (Product # PA5-102839) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-102839
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RAD21(Accession O60216), corresponding to amino acid residues K527-E577.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Rad21.
Target Information
This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ25655; FLJ40596; h NXP-1; HR21spA; KIAA0078; KIAA0078RAD21; kleisin; nuclear matrix protein 1; NXP-1; pokeweed agglutinin-binding protein 29; protein involved in DNA double-strand break repair; PW29; RAD21 homolog; SCC1 homolog; sister chromatid cohesion 1; unnamed protein product
Gene Aliases: CDLS4; hHR21; HR21; HRAD21; MCD1; MGS; mHR21; mKIAA0078; NXP1; SCC1
UniProt ID: (Human) O60216, (Mouse) Q61550
Entrez Gene ID: (Human) 5885, (Mouse) 19357
response to hypoxia
regulation of transcription from RNA polymerase II promoter
sister chromatid cohesion
positive regulation of gene expression
negative regulation of gene expression
negative regulation of G2/M transition of mitotic cell cycle
negative regulation of interleukin-1 beta production
negative regulation of tumor necrosis factor production
positive regulation of interleukin-10 production
negative regulation of glial cell apoptotic process
negative regulation of neuron apoptotic process
negative regulation of mitotic metaphase/anaphase transition
positive regulation of sister chromatid cohesion
protein localization to chromatin
chromatin looping
replication-born double-strand break repair via sister chromatid exchange
double-strand break repair
DNA recombination
reciprocal meiotic recombination
establishment of mitotic sister chromatid cohesion
establishment of meiotic sister chromatid cohesion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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