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Invitrogen

Phospho-MEKK1 (Thr1400) Polyclonal Antibody

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Phospho-MEKK1 (Thr1400) Polyclonal Antibody

Product Details

PA5-103271

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human MEKK1 around the phosphorylation site of Thr1402.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815970

Product Specific Information

Antibody detects endogenous levels of MEKK1 only when phosphorylated at Thr1400, which site historically referenced as Thr1402.

Target Information

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK1 can phosphorylate and activate MAPKK 1 and MAPKK 2 (MEK1/MEK2) which leads to phosphorylation of MAP kinases. It is also a highly efficient activator of the JNK cascade. The protein contains a putative 1 RING-type zinc finger and 1 SWIM-type zinc finger.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase; protein kinase MEKK1

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Gene Aliases: MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6

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UniProt ID: (Human) Q13233, (Rat) Q62925

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Entrez Gene ID: (Human) 4214, (Mouse) 26401, (Rat) 116667

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding zinc ion binding protein kinase binding ubiquitin protein ligase activity protein serine kinase activity JUN kinase kinase kinase activity MAP kinase kinase activity ubiquitin-protein transferase activity cytoskeletal protein binding JUN kinase binding mitogen-activated protein kinase kinase binding macromolecular complex binding sphingolipid binding mitogen-activated protein kinase binding
Process(es)
MAPK cascade protein phosphorylation cell surface receptor signaling pathway Fc-epsilon receptor signaling pathway cellular response to mechanical stimulus protein polyubiquitination morphogenesis of an epithelial sheet epithelial cell morphogenesis ubiquitin-dependent protein catabolic process transforming growth factor beta receptor signaling pathway JNK cascade apoptotic mitochondrial changes peptidyl-serine phosphorylation regulation of cell migration positive regulation of actin filament polymerization negative regulation of actin filament bundle assembly wound healing camera-type eye development positive regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of MAPK cascade positive regulation of JUN kinase activity positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade protein autophosphorylation positive regulation of viral transcription eyelid development in camera-type eye positive regulation of canonical Wnt signaling pathway regulation of actin filament organization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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