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Invitrogen
Phospho-PAK2 (Ser20) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-PAK2 (Ser20) Antibody (PA5-104629) in ICC/IF
Immunocytochemistry analysis of Phospho-PAK2 (Ser20) in Heat-shock treated Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-PAK2 (Ser20) polyclonal antibody (Product # PA5-104629) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104629
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PAK2(Accession Q13177), corresponding to amino acid residues around phosphorylated Ser20.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of PAK2 only when phosphorylated at Ser20.
Target Information
PAK2 (serine/threonine-protein kinase PAK2) is a serine/threonine kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, or proliferation. It acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformation change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-PAK; Beta-PAK; gamma pak; gamma-PAK; Oligophrenin-3; OPHN3; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p21cdc42/rac1-activated serine/threonine kinase; p58; p65-PAK; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK1; PAK3; S6/H4 kinase; serine/threonine-protein kinase PAK 2-like; unnamed protein product
Gene Aliases: 5330420P17Rik; A130002K10Rik; D16Ertd269e; gamma-PAK; KNO2; mKIAA4182; PAK-2; PAK65; PAKgamma
UniProt ID: (Human) Q13177, (Rat) Q64303, (Mouse) Q8CIN4
Entrez Gene ID: (Human) 5062, (Rat) 29432, (Mouse) 224105
cardiac muscle hypertrophy
protein phosphorylation
cellular response to starvation
cell migration
adherens junction assembly
intracellular signal transduction
negative regulation of apoptotic process
regulation of MAPK cascade
protein autophosphorylation
regulation of axonogenesis
regulation of cytoskeleton organization
negative regulation of stress fiber assembly
dendritic spine development
bicellular tight junction assembly
cellular response to transforming growth factor beta stimulus
protein localization to cell-cell junction
positive regulation of extrinsic apoptotic signaling pathway
stimulatory C-type lectin receptor signaling pathway
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
vascular endothelial growth factor receptor signaling pathway
regulation of actin filament organization
protein maturation
execution phase of apoptosis
positive regulation of execution phase of apoptosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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