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Invitrogen

Phospho-KLF4 (Ser254) Polyclonal Antibody

View all (28) KLF4 antibodies

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Phospho-KLF4 (Ser254) Polyclonal Antibody

Product Details

PA5-104680

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human KLF4(Accession O43474), corresponding to amino acid residues around phosphorylated Ser254.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816155

Product Specific Information

Antibody detects endogenous levels of KLF4 only when phosphorylated at Ser254.

Target Information

Kruppel like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. It is also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF) and belongs to a family of conserved Kruppel-like factor family of DNA binding zinc finger transcription factors. KLF4 exerts its functional relevance across varied cellular pathways. As one of the four Yamanaka factors, it plays a key role in reprogramming differentiated cells into pluripotent stem cells. Most studies have focused on its specific role in endothelial cell signaling, adipogenesis, differentiation, embryogenesis and cell cycle control. Localization plays an important role in the biological significance of this protein wherein nuclear localization is associated with its function as an oncogene and cytosolic localization with tumor suppression specific effect.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: endothelial Kruppel-like zinc finger protein; epithelial zinc finger protein EZF; gut Kruppel-like factor; gut-enriched Krueppel-like factor; Kruppel like factor 4; Kruppel-like factor 4 (Gut enriched kruppel-like factor) (Epithelial zinc-finger protein EZF); Kruppel-like factor 4 (gut); Kruppel-like zinc finger protein; RP11-150J11.1; transcription factor; unnamed protein product

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Gene Aliases: EZF; GKLF; Zie

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UniProt ID: (Human) O43474, (Mouse) Q60793

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Entrez Gene ID: (Human) 9314, (Rat) 114505, (Mouse) 16600

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding zinc ion binding chromatin DNA binding phosphatidylinositol 3-kinase regulator activity histone deacetylase binding sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding lncRNA binding sequence-specific double-stranded DNA binding promoter-specific chromatin binding DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter defense response to tumor cell transcription, DNA-templated regulation of transcription from RNA polymerase II promoter negative regulation of cell proliferation epidermis development epidermal cell differentiation gene expression positive regulation of gene expression negative regulation of gene expression negative regulation of muscle hyperplasia negative regulation of angiogenesis stem cell population maintenance cell differentiation negative regulation of cell migration post-embryonic camera-type eye development positive regulation of telomere maintenance response to retinoic acid negative regulation of interleukin-8 production negative regulation of heterotypic cell-cell adhesion post-embryonic hemopoiesis regulation of cell proliferation negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of nitric oxide biosynthetic process fat cell differentiation regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hemoglobin biosynthetic process negative regulation of smooth muscle cell proliferation regulation of axon regeneration epidermis morphogenesis positive regulation of protein metabolic process negative regulation of protein kinase B signaling canonical Wnt signaling pathway negative regulation of response to cytokine stimulus establishment of skin barrier cellular response to hydrogen peroxide negative regulation of ERK1 and ERK2 cascade cellular response to retinoic acid cellular response to growth factor stimulus cellular response to laminar fluid shear stress negative regulation of cell migration involved in sprouting angiogenesis regulation of blastocyst development positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of sprouting angiogenesis negative regulation of leukocyte adhesion to arterial endothelial cell cellular response to leukemia inhibitory factor cellular response to endothelin negative regulation of G1/S transition of mitotic cell cycle negative regulation of chemokine (C-X-C motif) ligand 2 production negative regulation of extrinsic apoptotic signaling pathway in absence of ligand mesodermal cell fate determination negative regulation of inflammatory response
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