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Invitrogen
Phospho-AKT1 (Ser129) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-AKT1 (Ser129) Antibody (PA5-104868) in WB
Western blot analysis of Phospho-AKT1 (Ser129) in extracts from various samples. Samples were incubated with Phospho-AKT1 (Ser129) polyclonal antibody (Product # PA5-104868). Lane 1: 3t3-l1 cells (heat-shock treatment), blocked with antigen-specific peptides; Lane 2: 3t3-l1 cells (heat-shock treatment); Lane 3: Mcf7 cells (heat-shock treatment); Lane 4: K562 cells (heat-shock treatment).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104868
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human AKT1(Accession P31749), corresponding to amino acid residues around phosphorylated Ser129.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of AKT1 only when phosphorylated at Ser129.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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