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Invitrogen
Phospho-NFkB p52/p100 (Ser870) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-NFkB p52/p100 (Ser870) Antibody (PA5-104947) in ICC/IF
Immunofluorescent analysis of Phospho-NFkB p100 (Ser869) in HepG2 cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-NFkB p100 (Ser869) polyclonal antibody (Product # PA5-104947) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104947
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NFKB2(Accession Q00653), corresponding to amino acid residues around phosphorylated Ser870.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of NF kappaB p100/p52 only when phosphorylated at Serine 870.
Target Information
The NFkB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFkB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-binding factor KBF2; lymphocyte translocation chromosome 10 protein; NF kappaB2; NF KB; NF-kB p52/p100; NF-kB2.v1; NF-kB2.v2; NF-kB2.v3; NF-kB2.v4; nfkb p100; NFKB, p52/p100 subunit; NFkB2; NFKB2 p100 subunit; nuclear factor; nuclear factor kappa b; nuclear factor Kappa-B, subunit 2; nuclear factor NF-kappa-B p52 subunit; nuclear factor of Kappa light chain gene enhancer in B cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100; oncogene Lyt-10; p50-NF-kappa B homolog; transcription factor NFKB2; unnamed protein product
Gene Aliases: CVID10; H2TF1; lyt; LYT-10; LYT10; NF-kappaB2; NF-kB2; p100; p49; p49/p100; p50B; p52; RGD1307189
UniProt ID: (Human) Q00653, (Mouse) Q9WTK5
Entrez Gene ID: (Human) 4791, (Rat) 309452, (Mouse) 18034
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
signal transduction
I-kappaB kinase/NF-kappaB signaling
response to lipopolysaccharide
response to cytokine
NIK/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
intracellular signaling cassette
follicular dendritic cell differentiation
germinal center formation
extracellular matrix organization
spleen development
lymph node development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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