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          • Primary Antibodies ›
          • IL-1 beta (Cleaved Asp116) Antibodies

          Invitrogen

          IL-1 beta (Cleaved Asp116) Polyclonal Antibody

          5 References

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          Cite IL-1 beta (Cleaved Asp116) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          IL-1 beta (Cleaved Asp116) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          IL-1 beta (Cleaved Asp116) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          IL-1 beta (Cleaved Asp116) Antibody (PA5-105048) in WB

          Western blot analysis of IL-1 beta (Cleaved Asp116) in mouse brain and mouse spleen. Samples were incubated with IL-1 beta (Cleaved Asp116) polyclonal antibody (Product # PA5-105048). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          IL-1 beta (Cleaved Asp116) Antibody in Western Blot (WB)
          IL-1 beta (Cleaved Asp116) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          PA5-105048

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat, Zebrafish

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human IL1B(Accession P01584), corresponding to amino acid residues N110-Q130.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2816521

          Product Specific Information

          Antibody detects endogenous levels of fragment of activated IL-1 beta resulting from cleavage adjacent to Asp116.

          Target Information

          IL-1 beta (Cleaved Asp116) refers to the mature, biologically active form of interleukin-1 beta generated after proteolytic processing of its inactive precursor, pro-IL-1 beta. IL-1 beta is encoded by the IL1B gene and is synthesized as a 31 kDa cytosolic precursor in response to inflammatory stimuli such as infection or tissue injury. Activation of inflammasome complexes leads to caspase-1-mediated cleavage at aspartic acid residue 116, producing the approximately 17 kDa mature cytokine. The cleaved form is released through non-classical secretion pathways and binds to the interleukin-1 receptor type 1 (IL-1R1), triggering downstream signaling through NF-kappa B and MAP kinase pathways. Mature IL-1 beta is a potent pro-inflammatory cytokine that promotes fever, leukocyte recruitment, and amplification of innate and adaptive immune responses. Detection of IL-1 beta cleaved at Asp116 is widely used as a marker of inflammasome activation and inflammatory signaling in immunology and disease research.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: catabolin; Cleaved IL-1 beta; IL-1; IL-1 beta; IL1B; interleukin 1 precursor polypeptide; interleukin 1b; interleukin 1beta; interlukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta; unnamed protein product

          View more View less

          Gene Aliases: IL-1; Il-1b; IL-1beta; IL-1F2; il1-b; IL1-BETA; IL1beta; IL1F2; zgc:111873

          View more View less

          UniProt ID: (Human) P01584, (Mouse) P10749

          View more View less

          Entrez Gene ID: (Human) 3553, (Rat) 24494, (Mouse) 16176, (Zebrafish) 405770

          View more View less

          Function(s)
          cytokine activity interleukin-1 receptor binding integrin binding protein binding protein domain specific binding receptor agonist activity interleukin-1 receptor antagonist activity interleukin superfamily
          Process(es)
          positive regulation of cytokine production microglial cell activation involved in immune response positive regulation of T cell mediated immunity apoptotic process inflammatory response immune response signal transduction cell-cell signaling embryo implantation positive regulation of cell proliferation negative regulation of cell proliferation vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of platelet-derived growth factor receptor signaling pathway positive regulation of epithelial to mesenchymal transition positive regulation of macrophage derived foam cell differentiation negative regulation of glucose transport smooth muscle adaptation cytokine-mediated signaling pathway hyaluronan biosynthetic process monocyte differentiation positive regulation of cell migration positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of prostaglandin biosynthetic process positive regulation of fever generation positive regulation of prostaglandin secretion response to lipopolysaccharide negative regulation of interleukin-1 beta production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of immature T cell proliferation in thymus positive regulation of heterotypic cell-cell adhesion positive regulation of T cell proliferation regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAPK cascade positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of complement activation positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway positive regulation of protein export from nucleus regulation of defense response to virus by host negative regulation of inflammatory response positive regulation of inflammatory response regulation of neurogenesis negative regulation of neurogenesis regulation of insulin secretion negative regulation of synaptic transmission defense response to Gram-positive bacterium negative regulation of lipid catabolic process positive regulation of lipid catabolic process regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis positive regulation of protein kinase B signaling positive regulation of glial cell proliferation positive regulation of cell adhesion molecule production negative regulation of adiponectin secretion regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade monocyte aggregation interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to xenobiotic stimulus positive regulation of monocyte chemotactic protein-1 production cellular response to interleukin-17 positive regulation of neuroinflammatory response positive regulation of p38MAPK cascade positive regulation of NIK/NF-kappaB signaling positive regulation of RNA biosynthetic process regulation of establishment of endothelial barrier regulation of secretion by cell negative regulation of gap junction assembly positive regulation of occluding junction disassembly positive regulation of T-helper 1 cell cytokine production negative regulation of interleukin-1-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of transcription from RNA polymerase II promoter fever generation response to hypoxia response to amphetamine chronic inflammatory response to antigenic stimulus positive regulation of cytosolic calcium ion concentration response to nutrient learning or memory memory response to xenobiotic stimulus response to carbohydrate response to ozone response to gamma radiation negative regulation of gene expression negative regulation of glutamate secretion neutrophil chemotaxis lipopolysaccharide-mediated signaling pathway response to estradiol positive regulation of chemokine production positive regulation of stress-activated MAPK cascade regulation of organic acid transport response to ATP response to vitamin D response to L-ascorbic acid social behavior ectopic germ cell programmed cell death response to stilbenoid response to isolation stress response to immobilization stress positive regulation of apoptotic process response to peptide hormone positive regulation of JUN kinase activity positive regulation of neuron apoptotic process response to ethanol negative regulation of neuron differentiation positive regulation of glial cell differentiation positive regulation of transcription from RNA polymerase II promoter astrocyte activation positive regulation of astrocyte differentiation leukocyte migration response to glucocorticoid cellular response to antibiotic cellular response to glucose stimulus cellular response to lipid cellular response to fatty acid response to dexamethasone positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand negative regulation of secretion by cell response to phorbol 13-acetate 12-myristate response to hydrogen sulfide negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation response to molecule of bacterial origin response to virus response to bacterium fin regeneration neutrophil activation defense response to bacterium response to exogenous dsRNA response to arsenic-containing substance macrophage chemotaxis regulation of NIK/NF-kappaB signaling
          It has to be done as per old AB suggested Products section.

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