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Invitrogen
Phospho-SMURF1 (Ser200) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-SMURF1 (Ser200) Antibody (PA5-105074) in WB
Western blot analysis of Phospho-SMURF1 (Ser200) in MCF7 (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-SMURF1 (Ser200) polyclonal antibody (Product # PA5-105074).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105074
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SMURF1(Accession Q9HCE7), corresponding to amino acid residues around phosphorylated Ser200.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of SMURF1 only when phosphorylated at Ser200.
Target Information
Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e. g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin ligase SMURF1; HECT-type E3 ubiquitin transferase SMURF1; hSMURF1; hSMURF2; Smad ubiquitination regulatory factor 1; Smad-specific E3 ubiquitin ligase 1; unnamed protein product
Gene Aliases: 4930431E10Rik; mKIAA1625; RGD1309707
UniProt ID: (Human) Q9HCE7, (Mouse) Q9CUN6
Entrez Gene ID: (Human) 57154, (Rat) 690516, (Mouse) 75788
protein polyubiquitination
ubiquitin-dependent protein catabolic process
protein export from nucleus
autophagy
ectoderm development
protein ubiquitination
cell differentiation
protein catabolic process
negative regulation of ossification
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
receptor catabolic process
negative regulation of activin receptor signaling pathway
negative regulation of MyD88-dependent toll-like receptor signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of osteoblast differentiation
positive regulation of axon extension
positive regulation of smoothened signaling pathway
defense response to virus
parkin-mediated mitophagy in response to mitochondrial depolarization
engulfment of target by autophagosome
substrate localization to autophagosome
protein K63-linked ubiquitination
protein targeting to vacuole involved in autophagy
protein localization to plasma membrane
positive regulation of dendrite extension
lysosomal protein catabolic process
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
Wnt signaling pathway, planar cell polarity pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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