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Invitrogen
Phospho-Fra1 (Ser265) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-Fra1 (Ser265) Antibody (PA5-105153) in ICC/IF
Immunocytochemistry analysis of Phospho-Fra1 (Ser265) in HepG2 cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-Fra1 (Ser265) polyclonal antibody (Product # PA5-105153) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105153
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human FOSL1(Accession P15407), corresponding to amino acid residues around phosphorylated Ser265.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of FRA1 only when phosphorylated at Ser265.
Target Information
The v-Fos oncogene was initially detected in two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. Members of the c-Fos gene family, including c-Fos, Fos B, Fra-1 and Fra-2, encode nuclear phosphoproteins that are rapidly and transiently induced by a variety of agents and function as transcriptional regulators for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers that bind to specific DNA response elements, c-Fos proteins are only active as heterodimers with members of the Jun gene family. In addition, selected ATF/CREB family members can form leucine zipper dimers with Fos and Jun. Different dimers exhibit differential specificity and affinity for AP-1 and CRE sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FOS like 1, AP-1 trancription factor subunit; fos-like antigen 1; FOS-like antigen-1; fos-related antigen; FOSL1; HGNC:13718; unnamed protein product
Gene Aliases: FRA; Fra-1; FRA1
UniProt ID: (Human) P15407, (Mouse) P48755, (Rat) P10158
Entrez Gene ID: (Human) 8061, (Mouse) 14283, (Rat) 25445
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
double-stranded DNA binding
in utero embryonic development
toll-like receptor signaling pathway
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
inflammatory response
vitellogenesis
negative regulation of cell proliferation
response to wounding
gene expression
cytokine-mediated signaling pathway
positive regulation of apoptotic process
positive regulation of cell cycle
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
placenta blood vessel development
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of DNA-templated transcription, initiation
female pregnancy
learning
response to xenobiotic stimulus
response to mechanical stimulus
response to gravity
response to progesterone
response to cytokine
response to hydrogen peroxide
response to corticosterone
response to cAMP
response to forskolin
chemotaxis
cellular defense response
positive regulation of cell proliferation
response to virus
integrated stress response signaling
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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