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Invitrogen
Caspase 9 (Cleaved Asp330) Polyclonal Antibody
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FIGURE: 1 / 4
Caspase 9 (Cleaved Asp330) Antibody (PA5-105272) in ICC/IF
Immunocytochemistry analysis of Caspase 9 (Cleaved Asp330) in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Caspase 9 (Cleaved Asp330) polyclonal antibody (Product # PA5-105272) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105272
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CASP9(Accession P55211), corresponding to amino acid residues E313-S339.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp330.
Target Information
Caspase 9 (Cleaved Asp330) encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: apoptotic protease activating factor 3; apoptotic protease MCH-6; CASP9; caspase 9, apoptosis-related cysteine peptidase; caspase 9b; caspase-9 variant; ICE-like apoptotic protease 6; protein phosphatase 1, regulatory subunit 56; unnamed protein product
Gene Aliases: APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56
UniProt ID: (Human) P55211
Entrez Gene ID: (Human) 842
response to hypoxia
kidney development
response to ischemia
proteolysis
apoptotic process
cellular response to DNA damage stimulus
intrinsic apoptotic signaling pathway in response to DNA damage
response to UV
protein processing
platelet formation
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
cellular response to UV
signal transduction in response to DNA damage
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
response to ethanol
protein maturation
cellular response to dexamethasone stimulus
response to indole-3-methanol
leukocyte apoptotic process
response to anesthetic
intrinsic apoptotic signaling pathway
positive regulation of execution phase of apoptosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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