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Invitrogen

Phospho-MYOD (Thr115) Polyclonal Antibody

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Phospho-MYOD (Thr115) Polyclonal Antibody

Product Details

PA5-105502

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human MYOD1(Accession P15172), corresponding to amino acid residues around phosphorylated Thr115.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816930

Product Specific Information

Antibody detects endogenous levels of MyoD1 only when phosphorylated at Thr115.

Target Information

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: class C basic helix-loop-helix protein 1; myf-3; MyoD; MyoD1; myogenic factor 3; unnamed protein product

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Gene Aliases: bHLHc1; CMYO17; CMYP17; MYF3; MYOD; Myod-1; MYODRIF; PUM

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UniProt ID: (Human) P15172, (Rat) Q02346, (Mouse) P10085

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Entrez Gene ID: (Human) 4654, (Rat) 337868, (Mouse) 17927

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding ligand-dependent nuclear receptor binding enzyme binding chromatin DNA binding ubiquitin protein ligase binding protein homodimerization activity bHLH transcription factor binding sequence-specific DNA binding protein dimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding sequence-specific double-stranded DNA binding promoter-specific chromatin binding
Process(es)
regulation of alternative mRNA splicing, via spliceosome regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter muscle organ development myoblast fate determination skeletal muscle tissue development myoblast fusion cellular response to starvation regulation of gene expression skeletal muscle atrophy myotube differentiation myotube cell development myotube differentiation involved in skeletal muscle regeneration skeletal muscle cell differentiation response to muscle stretch muscle cell differentiation protein unfolding skeletal muscle tissue regeneration positive regulation of skeletal muscle tissue regeneration regulation of RNA splicing skeletal muscle fiber adaptation myoblast differentiation positive regulation of myoblast differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter skeletal muscle fiber development positive regulation of skeletal muscle fiber development striated muscle cell differentiation positive regulation of muscle cell differentiation cellular response to tumor necrosis factor cellular response to glucocorticoid stimulus cellular response to estradiol stimulus cellular response to oxygen levels positive regulation of myoblast fusion positive regulation of snRNA transcription by RNA polymerase II negative regulation of myoblast proliferation tissue development muscle cell fate commitment animal organ development cardiac muscle cell differentiation
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