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Invitrogen

Phospho-GRK2 (Tyr86) Polyclonal Antibody

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Phospho-GRK2 (Tyr86) Polyclonal Antibody

Product Details

PA5-106089

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human GRK2(Accession P25098), corresponding to amino acid residues around phosphorylated Tyr86.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2817488

Product Specific Information

Antibody detects endogenous levels of GRK2 only when phosphorylated at Tyr86.

Target Information

ADRBK1 (GRK2) is a serine/threonine kinase. Many of the G protein-coupled receptors are known to be phosphorylated by G protein-dependent receptor kinases (GRKs). G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: adrenergic beta receptor kinase 1; adrenergic receptor kinase, beta 1; adrenergic, beta, receptor kinase 1; beta-adrenergic receptor kinase 1; beta-adrenergic receptor kinase 1 beta ARK1; beta-AR kinase-1; beta-ARK-1; EC 2.7.1.126; grk 2; GRK2; unnamed protein product

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Gene Aliases: Adrbk-1; ADRBK1; Bark-1; BARK1; beta ARK; BETA-ARK1; betaARK1; GRK-2

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UniProt ID: (Human) P25098, (Mouse) Q99MK8, (Rat) P26817

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Entrez Gene ID: (Human) 156, (Mouse) 110355, (Rat) 25238

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Function(s)
G-protein coupled receptor binding protein kinase activity protein serine/threonine kinase activity G-protein coupled receptor kinase activity protein binding ATP binding alpha-2A adrenergic receptor binding Edg-2 lysophosphatidic acid receptor binding beta-adrenergic receptor kinase activity ubiquitin protein ligase binding delta-type opioid receptor binding kappa-type opioid receptor binding scaffold protein binding
Process(es)
regulation of the force of heart contraction desensitization of G-protein coupled receptor protein signaling pathway negative regulation of the force of heart contraction by chemical signal protein phosphorylation response to oxidative stress signal transduction G-protein coupled receptor signaling pathway tachykinin receptor signaling pathway heart development positive regulation of muscle cell apoptotic process negative regulation of peptidyl-threonine phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation viral genome replication receptor internalization positive regulation of interleukin-6 production regulation of inositol trisphosphate biosynthetic process negative regulation of peptidyl-serine phosphorylation positive regulation of catecholamine secretion intraciliary retrograde transport vasodilation follicle-stimulating hormone signaling pathway negative regulation of apoptotic process negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of smoothened signaling pathway positive regulation of vasoconstriction negative regulation of striated muscle contraction negative regulation of glucose import viral entry into host cell positive regulation of fibroblast proliferation oligodendrocyte differentiation maintenance of protein location in nucleus cardiac muscle contraction positive regulation of ERK1 and ERK2 cascade cellular response to glucose stimulus cellular response to epidermal growth factor stimulus negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway negative regulation of cellular response to insulin stimulus negative regulation of relaxation of smooth muscle negative regulation of protein localization to ciliary membrane positive regulation of vascular smooth muscle cell proliferation positive regulation of intraciliary retrograde transport cellular response to chemokine positive regulation of excitatory postsynaptic potential negative regulation of apoptotic signaling pathway G-protein coupled acetylcholine receptor signaling pathway response to muscle activity response to hydrogen peroxide response to leptin response to caloric restriction cellular response to catecholamine stimulus cellular response to rapamycin cellular response to phorbol 13-acetate 12-myristate
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