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Invitrogen
MICAL1 Polyclonal Antibody
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FIGURE: 1 / 4
MICAL1 Antibody (PA5-106316) in ICC/IF
Immunofluorescent analysis of MICAL1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and MICAL1 polyclonal antibody (Product # PA5-106316) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-106316
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MICAL1(Accession Q8TDZ2), corresponding to amino acid residues E437-A487.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total MICAL1.
Target Information
May be a cytoskeletal regulator that connects NEDD9 to intermediate filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [F-actin]-methionine sulfoxide oxidase MICAL1; CasL interacting molecule MICAL; F-actin-monooxygenase MICAL1; microtubule associated monoxygenase, calponin and LIM domain containing 1; molecule interacting with CasL protein 1; NEDD9-interacting protein with calponin homology and LIM domains; protein-methionine sulfoxide oxidase MICAL1; unnamed protein product
Gene Aliases: MICAL; MICAL-1; NICAL
UniProt ID: (Human) Q8TDZ2, (Mouse) Q8VDP3
Entrez Gene ID: (Human) 64780, (Mouse) 171580
actin binding
monooxygenase activity
protein binding
NAD(P)H oxidase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
SH3 domain binding
protein kinase binding
small GTPase binding
actin filament binding
FAD binding
F-actin monooxygenase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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