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Invitrogen
TEF Polyclonal Antibody
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FIGURE: 1 / 2
TEF Antibody (PA5-106495) in ICC/IF
Immunofluorescent analysis of TEF in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TEF polyclonal antibody (Product # PA5-106495) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-106495
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TEF(Accession Q10587), corresponding to amino acid residues R204-R254.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TEF.
Target Information
TEF (thyrotrophic embryonic factor), also known as KIAA1655, is a 303 amino acid nuclear transcription factor that belongs to the bZIP (basic region/leucine zipper) family and PAR (proline and acidic amino acid-rich) subfamily. TEF binds DNA as either a homodimer or heterodimer, and is known to transactivate the TSH beta promoter. While broadly expressed in adults, TEF is only found in developing embryonic anterior pituitary gland. TEF accumulates according to a robust circadian rhythm and has also been found to inhibit cell growth by downregulating beta chain expression of cytokine receptors. The functional domains of TEF are highly homologous with other members of the PAR-bZIP subfamily, including albumin D box-binding protein (DABP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: leucine zipper transcription factor; TEF; TEF, PAR bZIP transcription factor; TEF; PAR-bZIP transcription factor; thyrotrophic embryonic factor; transcription factor; unnamed protein product
Gene Aliases: 2310028D20Rik
UniProt ID: (Human) Q10587, (Mouse) Q9JLC6, (Rat) P41224
Entrez Gene ID: (Human) 7008, (Mouse) 21685, (Rat) 29362
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
identical protein binding
sequence-specific DNA binding
macromolecular complex binding
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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