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Invitrogen
ALY Polyclonal Antibody
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FIGURE: 1 / 3
ALY Antibody (PA5-106782) in ICC/IF
Immunocytochemistry analysis of ALY in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ALY polyclonal antibody (Product # PA5-106782) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-106782
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ALYREF(Accession Q86V81), corresponding to amino acid residues F129-Q179.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total THOC4.
Target Information
The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ally of AML-1 and LEF-1; bZIP enhancing factor; bZIP-enhancing factor BEF; coactivator of LEF-1 and AML-1; RNA and export factor binding protein 1; Tcra enhancer-binding factor interacting protein 1; THO complex 4; tho4; transcriptional coactivator Aly/REF; unnamed protein product
Gene Aliases: ALY; ALY/REF; BEF; REF; Ref1; Ref1-I; Refbp1; Tho4; THOC4
UniProt ID: (Human) Q86V81, (Mouse) O08583
Entrez Gene ID: (Human) 10189, (Mouse) 21681, (Rat) 690585
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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