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Invitrogen
GPR30 Polyclonal Antibody
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FIGURE: 1 / 5
GPR30 Antibody (PA5-109319) in ICC/IF
Immunofluorescent analysis of G-protein coupled receptor 30 in SH-SY-5Y cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with G-protein coupled receptor 30 polyclonal antibody (Product # PA5-109319) at a dilution of 1:100 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-109319
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human G-protein coupled receptor 30 aa 236-375.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Lovo, HepG2, MG-63, SH-SY-5Y, rat uterus tissue, human liver cancer tissue, human appendix tissue, human placenta tissue, mouse kidney tissue.
Target Information
This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: chemoattractant receptor-like 2; chemokine receptor-like 2; constitutively expressed peptide-like receptor; flow-induced endothelial G-protein coupled receptor 1; G protein-coupled receptor 30; GPR30; heptahelix receptor; IL8-related receptor DRY12; leucine rich protein in GPR30 3'UTR; leucine rich protein in GPR30 3'UTR (LERGU); lymphocyte-derived G-protein coupled receptor; membrane estrogen receptor; unnamed protein product
Gene Aliases: CEPR; CMKRL2; DRY12; FEG-1; GPCR-Br; GPER; GPR30; LERGU; LERGU2; LyGPR; mER
UniProt ID: (Human) Q99527
Entrez Gene ID: (Human) 2852
positive regulation of protein phosphorylation
positive regulation of neurotransmitter secretion
negative regulation of leukocyte activation
cell communication
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of gene expression
negative regulation of cell cycle process
neuronal action potential
apoptotic chromosome condensation
nuclear fragmentation involved in apoptotic nuclear change
positive regulation of cell migration
intracellular steroid hormone receptor signaling pathway
intracellular estrogen receptor signaling pathway
positive regulation of insulin secretion
positive regulation of inositol trisphosphate biosynthetic process
vasodilation
positive regulation of apoptotic process
steroid hormone mediated signaling pathway
positive regulation of MAPK cascade
negative regulation of fat cell differentiation
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
positive regulation of neurogenesis
modulation of synaptic transmission
positive regulation of transport
negative regulation of lipid biosynthetic process
positive regulation of release of sequestered calcium ion into cytosol
regulation of cytosolic calcium ion concentration
regulation of cell cycle
positive regulation of protein kinase B signaling
negative regulation of protein kinase B signaling
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of uterine smooth muscle contraction
cellular response to glucose stimulus
cellular response to tumor necrosis factor
cellular response to peptide hormone stimulus
cellular response to mineralocorticoid stimulus
cellular response to estradiol stimulus
positive regulation of release of cytochrome c from mitochondria
positive regulation of protein localization to plasma membrane
negative regulation of vascular smooth muscle cell proliferation
positive regulation of endothelial cell apoptotic process
positive regulation of cardiac vascular smooth muscle cell differentiation
positive regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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