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Invitrogen
HOIP Polyclonal Antibody
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FIGURE: 1 / 2
HOIP Antibody (PA5-114380) in WB
Western blot analysis of HOIP in the following samples: Lane 1: human placenta tissues lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human HeLA whole cell lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) an... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114380
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human RNF31/HOIP recombinant protein (Position: R970-K1072).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissues, human HepG2 whole cell, human PC-3 whole cell, human A549 whole cell, human U2OS whole cell, human Caco-2 whole cell, human Hela whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Lyn proto oncogene (Lcl/Yes related novel protein tyrosine kinase) is encoded by the Lyn gene located on chromosome 8 in humans and belongs to the Src family kinase. It is a non-receptor tyrosine kinase that acts as a mediator in transducing cellular signaling. The most well studied expression of Lyn is in hematopoietic cell types, both lymphoid and myeloid cells with exclusion in T lymphocytes. It has also been observed that Lyn plays diverse roles such as in regulation of B cell receptor signaling, mast cell signaling and Epithelial - Mesenchymal Transition (EMT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E3 ubiquitin ligase; FLJ23501; HOIL-1-interacting protein; MGC19975; putative Ariadne-like ubiquitin ligase; RING-type E3 ubiquitin transferase RNF31; unnamed protein product; zinc in-between-RING-finger ubiquitin-associated domain protein
Gene Aliases: Flj10111; HOIP; IMD115; mFLJ00217; Paul; ZIBRA
UniProt ID: (Human) Q96EP0, (Mouse) Q924T7
Entrez Gene ID: (Human) 55072, (Rat) 364386, (Mouse) 268749
protein polyubiquitination
protein ubiquitination
CD40 signaling pathway
defense response to bacterium
positive regulation of I-kappaB kinase/NF-kappaB signaling
T cell receptor signaling pathway
negative regulation of necroptotic process
protein linear polyubiquitination
positive regulation of xenophagy
cell surface receptor signaling pathway
I-kappaB kinase/NF-kappaB signaling
tumor necrosis factor-mediated signaling pathway
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of establishment of protein localization to mitochondrion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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