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Invitrogen
ONECUT2 Polyclonal Antibody
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FIGURE: 1 / 1
ONECUT2 Antibody (PA5-114396) in WB
Western blot analysis of ONECUT2 in the following samples: Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Tween (3 times, 5 minutes/wash) and blocked with 5% Non-fat Milk/TBS (1.5 hrs., room temperature). The membrane was incubated in ON... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114396
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human ONECUT2 (MKAAYTAYRCLTKDLEGCAMNPELTMESLGTLH) (aa 1-33).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human HEK293 whole cell, human HepG2 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
ONECUT2 is a member of the transcription factors of the ONECUT class, whose prototype is hepatocyte nuclear factor (HNF)-6. The distribution of OC-2 mRNA in humans is tissue-restricted, the strongest expression being detected in the liver and skin. ONECUT2 participates in the network of transcription factors required for liver differentiation and metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hepatocyte nuclear factor 6-beta; HNF-6-beta; HNF-6B; one cut homeobox 2; onecut 2; ONECUT-2 homeodomain transcription factor; transcription factor ONECUT-2; transcription factor ONECUT2
Gene Aliases: C730009D12; OC-2; OC2
UniProt ID: (Human) O95948, (Mouse) Q6XBJ3
Entrez Gene ID: (Human) 9480, (Mouse) 225631
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific double-stranded DNA binding
liver development
regulation of cell-matrix adhesion
epithelial cell development
regulation of transcription from RNA polymerase II promoter
anatomical structure morphogenesis
positive regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
pancreas development
endocrine pancreas development
cell fate commitment
positive regulation of transcription from RNA polymerase II promoter
peripheral nervous system neuron development
cilium morphogenesis
organ morphogenesis
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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