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Invitrogen
TERT Polyclonal Antibody
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FIGURE: 1 / 4
TERT Antibody (PA5-11446) in ICC/IF
Immunocytochemistry analysis of TERT in HeLa cells. Samples were incubated with TERT polyclonal antibody (Product # PA5-11446) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). TERT im... View More
Product Details
PA5-11446
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 627-656 amino acids from the central region of human TERT
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
800 µg
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase (TERT) have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HEST2; hTERT; Telomerase catalytic subunit; Telomerase reverse transcriptase; Telomerase-associated protein 2; TP2; unnamed protein product
Gene Aliases: CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TERT; TP2; TRT
UniProt ID: (Human) O14746
Entrez Gene ID: (Human) 7015
tRNA binding
transcription coactivator binding
DNA binding
telomerase activity
RNA binding
RNA-directed DNA polymerase activity
RNA-directed RNA polymerase activity
protein binding
transferase activity
nucleotidyltransferase activity
DNA polymerase activity
telomeric DNA binding
identical protein binding
protein homodimerization activity
metal ion binding
chaperone binding
telomerase RNA binding
template-free RNA nucleotidyltransferase
telomere maintenance via recombination
telomere maintenance
transcription, RNA-templated
RNA-dependent DNA biosynthetic process
protein import into nucleus
telomere maintenance via telomerase
mitochondrion organization
heart development
DNA strand elongation
positive regulation of Wnt signaling pathway
production of siRNA involved in RNA interference
regulation of protein stability
positive regulation of hair cycle
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of angiogenesis
positive regulation of glucose import
response to cadmium ion
establishment of protein localization to telomere
cellular response to hypoxia
DNA biosynthetic process
replicative senescence
siRNA transcription
positive regulation of G1/S transition of mitotic cell cycle
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of transdifferentiation
positive regulation of vascular smooth muscle cell proliferation
positive regulation of protein localization to nucleolus
positive regulation of vascular associated smooth muscle cell migration
negative regulation of endothelial cell apoptotic process
positive regulation of stem cell proliferation
negative regulation of cellular senescence
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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