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Invitrogen
CIDEA Polyclonal Antibody
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FIGURE: 1 / 3
CIDEA Antibody (PA5-115020) in ICC/IF
Immunocytochemistry analysis of CIDEA in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115020). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CIDEA(Accession O60543), corresponding to amino acid residues S20-T70.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CIDEA.
Target Information
This gene encodes the homolog of the mouse protein Cidea that has been shown to activate apoptosis. This activation of apoptosis is inhibited by the DNA fragmentation factor DFF45 but not by caspase inhibitors. Mice that lack functional Cidea have higher metabolic rates, higher lipolysis in brown adipose tissue and higher core body temperatures when subjected to cold. These mice are also resistant to diet-induced obesity and diabetes. This suggests that in mice this gene product plays a role in thermogenesis and lipolysis. Alternatively spliced transcripts have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell death activator CIDE-A; cell death-inducing DNA fragmentation factor, alpha subunit-like effector A; cide a; unnamed protein product
Gene Aliases: CIDE-A
UniProt ID: (Human) O60543
Entrez Gene ID: (Human) 1149, (Rat) 291541
temperature homeostasis
negative regulation of cytokine production
lipid metabolic process
apoptotic process
biological_process
lipid storage
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of tumor necrosis factor production
response to stilbenoid
fat cell differentiation
negative regulation of lipid catabolic process
negative regulation of multicellular organismal process
cellular response to cold
intermembrane lipid transfer
negative regulation of cold-induced thermogenesis
lipid droplet fusion
negative regulation of execution phase of apoptosis
regulation of apoptotic DNA fragmentation
positive regulation of transcription, DNA-templated
positive regulation of cold-induced thermogenesis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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