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Invitrogen
Bombesin Polyclonal Antibody
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FIGURE: 1 / 5
Bombesin Antibody (PA5-115272) in ICC/IF
Immunocytochemistry analysis of Bombesin in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115272) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115272
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GRP(Accession P07492), corresponding to amino acid residues L85-G135.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Gastrin Releasing Peptide.
Target Information
This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. Its preproprotein, following cleavage of a signal peptide, is further processed to produce either the 27 aa gastrin-releasing peptide or the 10 aa neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bombesin; neuromedin C; pre-progastrin releasing peptide; prepro-GRP; preprogastrin-releasing peptide; testicular tissue protein Li 103; unnamed protein product
Gene Aliases: BLP; BN; GRP-10; preproGRP; proGRP
UniProt ID: (Human) P07492, (Mouse) Q8R1I2, (Rat) P24393
Entrez Gene ID: (Human) 2922, (Mouse) 225642, (Rat) 171101
ossification
neuropeptide signaling pathway
regulation of gene expression
social behavior
psychomotor behavior
response to external biotic stimulus
mast cell degranulation
negative regulation of transmission of nerve impulse
positive regulation of peptide hormone secretion
positive regulation of phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of respiratory gaseous exchange
positive regulation of vascular associated smooth muscle cell apoptotic process
positive regulation of behavioral fear response
signal transduction
cellular response to sodium phosphate
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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