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Invitrogen
KLF5 Polyclonal Antibody
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FIGURE: 1 / 3
KLF5 Antibody (PA5-115480) in ICC/IF
Immunocytochemistry analysis of KLF5 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115480) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115480
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KLF5(Accession Q13887), corresponding to amino acid residues F278-Y328.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total KLF5.
Target Information
KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. It contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. KLF5 is a transcription activator that promotes cell proliferation and oncogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: (intestinal Kruppel-like factor; basic transcription element binding protein 2; basic transcription element binding protein BTEB2; basic transcription element-binding protein 2; BTE-binding protein 2; colon krueppel-like factor; colon kruppel-like factor; epididymis secretory sperm binding protein; GC box binding protein 2; intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; Kruppel like factor 5; Kruppel-like factor 5; Kruppel-like factor 5 (intestinal); RP11-505F3.5; testis-specific transcript; tKLF5; transcription factor; transcription factor BTEB2; unnamed protein product
Gene Aliases: 4930520J07Rik; BTEB2; CKLF; IKLF
UniProt ID: (Human) Q13887, (Mouse) Q9Z0Z7
Entrez Gene ID: (Human) 688, (Rat) 84410, (Mouse) 12224
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
MRF binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
regulation of transcription from RNA polymerase II promoter
positive regulation of cell proliferation
gene expression
regulation of gene expression
skeletal muscle satellite cell differentiation
satellite cell activation involved in skeletal muscle regeneration
myotube differentiation involved in skeletal muscle regeneration
skeletal muscle cell differentiation
skeletal muscle tissue regeneration
positive regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
intestinal epithelial cell development
positive regulation of transcription by transcription factor localization
cellular response to leukemia inhibitory factor
cellular response to endothelin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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