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Invitrogen
NOD3 Polyclonal Antibody
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FIGURE: 1 / 2
NOD3 Antibody (PA5-115658) in ICC/IF
Immunocytochemistry analysis of NOD3 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115658) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115658
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NLRC3(Accession Q7RTR2), corresponding to amino acid residues L1026-M1065.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NLRC3.
Target Information
NOD3 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD3 also known as NLR family CARD containing 3 (NLRC3), is predominantly expressed in the immune system, particularly in T lymphocytes, and its expression is strongly down-regulated following stimulation of the T-cell receptor complex and CD28, suggesting that NOD3 plays a role in attenuating the activation of T cells. NOD3 inhibits NF-βB, AP-1 and NFAT transcriptional activation in Jurkat T cells downstream of CD3/CD28 stimulation or treatment with PMA/ionomycin and decreases IL-2 and CD25 mRNA induction in activated cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CARD15-like protein; caterpillar 16.2; caterpiller protein 16.2; CLR16.2; NACHT, LRR and CARD domains-containing protein 3; NLR family, CARD domain containing 3, pseudogene 1; NOD-like receptor C3; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3; unnamed protein product
Gene Aliases: CLR16.2; D230007K08Rik; mFLJ00348; Nlrc3-ps1; NOD3; RGD1304902
UniProt ID: (Human) Q7RTR2, (Mouse) Q5DU56
Entrez Gene ID: (Human) 197358, (Rat) 287070, (Mouse) 268857
negative regulation of immune system process
I-kappaB kinase/NF-kappaB signaling
negative regulation of interferon-alpha production
negative regulation of interferon-beta production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
T cell activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
regulation of innate immune response
negative regulation of innate immune response
negative regulation of fibroblast proliferation
negative regulation of epithelial cell proliferation
negative regulation of inflammatory response
negative regulation of protein kinase B signaling
negative regulation of cytokine production involved in inflammatory response
negative regulation of NLRP3 inflammasome complex assembly
negative regulation of NIK/NF-kappaB signaling
regulation of protein ubiquitination
response to lipopolysaccharide
negative regulation of interleukin-1 beta production
negative regulation of interleukin-12 production
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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