Search

  • Order Status
  • Quick Order
  • Promos

Invitrogen

PDPK1 Polyclonal Antibody

View all (45) PDPK1 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Datasheet
Certificates
Protocols
Questions & Answers
Datasheet
Certificates
Protocols
Questions & Answers
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
PDPK1 Polyclonal Antibody

Product Details

PA5-115733

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PDPK1(Accession O15530), corresponding to amino acid residues L350-S400.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2900368

Product Specific Information

Antibody detects endogenous levels of total PDPK1.

Target Information

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: HGNC:8809; isoenzyme 1; kinase isoenzyme 1; lipoamide; OTTHUMP00000174525; OTTHUMP00000205076; PDK1; PDPK1; Pkb kinase; PkB kinase like gene 1; PkB-like 1; predicted protein of HQ0461; protein kinase B kinase; unnamed protein product

View more View less

Gene Aliases: PDK1; PRO0461

View more View less

UniProt ID: (Human) O15530, (Mouse) Q9Z2A0, (Rat) O55173

View more View less

Entrez Gene ID: (Human) 5170, (Mouse) 18607, (Rat) 81745

View more View less

Function(s)
protein kinase activity protein serine/threonine kinase activity 3-phosphoinositide-dependent protein kinase activity protein binding ATP binding phospholipase activator activity phospholipase binding protein serine kinase activity insulin receptor binding protein kinase binding
Process(es)
type B pancreatic cell development hyperosmotic response signal transduction epidermal growth factor receptor signaling pathway insulin receptor signaling pathway regulation of endothelial cell migration negative regulation of cardiac muscle cell apoptotic process cell migration calcium-mediated signaling negative regulation of transforming growth factor beta receptor signaling pathway cellular response to insulin stimulus negative regulation of toll-like receptor signaling pathway intracellular signal transduction negative regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling regulation of mast cell degranulation protein kinase B signaling negative regulation of neuron apoptotic process positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis insulin-like growth factor receptor signaling pathway focal adhesion assembly positive regulation of release of sequestered calcium ion into cytosol positive regulation of protein kinase B signaling cellular response to epidermal growth factor stimulus extrinsic apoptotic signaling pathway intracellular signaling cassette positive regulation of protein localization to plasma membrane positive regulation of sprouting angiogenesis positive regulation of vascular endothelial cell proliferation negative regulation of endothelial cell apoptotic process protein phosphorylation actin cytoskeleton organization T cell costimulation protein autophosphorylation vascular endothelial cell response to laminar fluid shear stress cellular response to brain-derived neurotrophic factor stimulus
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support