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Invitrogen

Nhe-1 Polyclonal Antibody

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Nhe-1 Polyclonal Antibody

Product Details

PA5-115917

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SLC9A1(Accession P19634), corresponding to amino acid residues R516-E566.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2900551

Product Specific Information

Antibody detects endogenous levels of total SLC9A1.

Target Information

The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Na(+)/H(+) exchanger 1; Na+/H+ antiporter; Na+/H+, amiloride sensitive; Na-Li countertransporter; NHE-1; plasma membrane antiporter; hemopoietic NHE1; APNH; protein phosphatase 1, regulatory subunit 143; slow-wave epilepsy; Solute carrier family 9 (sodium/hydrogen exchanger 1), antiporter, Na+/H+, (amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), member 1; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 member 1; solute carrier family 9, member 1; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1; unnamed protein product

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Gene Aliases: APNH; LIKNS; mir-5122; Mir5122; NHE-1; NHE1; PPP1R143; swe

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UniProt ID: (Human) P19634, (Mouse) Q61165, (Rat) P26431

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Entrez Gene ID: (Human) 6548, (Mouse) 20544, (Rat) 24782

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Function(s)
calcium ion binding protein binding phospholipid binding phosphatidylinositol-4,5-bisphosphate binding antiporter activity sodium:proton antiporter activity potassium:proton antiporter activity protein phosphatase 2B binding protein binding, bridging identical protein binding calcium-dependent protein binding binding, bridging
Process(es)
regulation of the force of heart contraction cation transport sodium ion transport cellular sodium ion homeostasis regulation of pH response to acidic pH positive regulation of cardiac muscle hypertrophy regulation of cardiac muscle contraction by calcium ion signaling cell differentiation hyaluronan catabolic process positive regulation of cell growth cellular response to insulin stimulus positive regulation of mitochondrial membrane permeability response to muscle stretch sodium ion export from cell positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of action potential positive regulation of transcription from RNA polymerase II promoter protein oligomerization regulation of intracellular pH cardiac muscle cell differentiation transmembrane transport cellular response to cold positive regulation of calcineurin-NFAT signaling cascade cellular response to antibiotic cellular response to electrical stimulus cellular response to hypoxia cellular response to acidic pH potassium ion transmembrane transport cellular response to epinephrine stimulus cardiac muscle cell contraction regulation of the force of heart contraction by cardiac conduction sodium ion import across plasma membrane positive regulation of the force of heart contraction positive regulation of intracellular signal transduction hydrogen ion transmembrane transport ion transport cell migration maintenance of cell polarity sodium ion transmembrane transport regulation of stress fiber assembly regulation of focal adhesion assembly cellular response to mechanical stimulus regulation of cardiac muscle cell membrane potential
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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