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Invitrogen
SPRED2 Polyclonal Antibody
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FIGURE: 1 / 4
SPRED2 Antibody (PA5-115958) in ICC/IF
Immunocytochemistry analysis of SPRED2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115958) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115958
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SPRED2(Accession Q7Z698), corresponding to amino acid residues R298-S348.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total SPRED2.
Target Information
SPRED2 is a member of the Sprouty /SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (Nonami et al., 2004 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EVH1/Sprouty domain containing protein; spred-2; sprouty protein with EVH-1 domain 2, related sequence; unnamed protein product
Gene Aliases: NS14; Spred-2
UniProt ID: (Human) Q7Z698, (Mouse) Q924S7, (Rat) Q3C2P8
Entrez Gene ID: (Human) 200734, (Mouse) 114716, (Rat) 305539
regulation of signal transduction
negative regulation of epithelial to mesenchymal transition
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of MAPK cascade
positive regulation of DNA damage response, signal transduction by p53 class mediator
negative regulation of ERK1 and ERK2 cascade
negative regulation of intracellular signal transduction
negative regulation of lens fiber cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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