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Invitrogen
TRADD Polyclonal Antibody
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FIGURE: 1 / 3
TRADD Antibody (PA5-116447) in IHC (P)
Immunohistochemical analysis of TRADD in a paraffin-embedded human kidney tissue using a TRADD polyclonal antibody (Product #PA5-116447). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1:50 dilutio... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-116447
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human TRADD aa 109-312.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive controls: Mouse spleen tissue lysates, rat uterus tissue, human kidney tissue.
Target Information
TRADD (Tumor necrosis factor receptor type 1-associated DEATH domain protein) is an important mediator of FAS and TNFR1 associated apoptotic signaling. The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12. TRADD acts by interacting with TRIP12 and leading to the disruption of the interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A. TRADD acts as an adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interation with FADD. Overexpression of TRADD leads to two major TNF-induced responses - apoptosis and activation of NF-kappa-B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hs89862; MGC11078; TNF receptor 1 associated signal transducer; TNFR1-associated DEATH domain protein; TRADD; tumor necrosis factor receptor type 1 associated death domain protein; tumor necrosis factor receptor-1-associated protein; unnamed protein product
Gene Aliases: 9130005N23Rik; Hs.89862
UniProt ID: (Human) Q15628, (Mouse) Q3U0V2
Entrez Gene ID: (Human) 8717, (Rat) 246756, (Mouse) 71609
apoptotic process
inflammatory response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
I-kappaB kinase/NF-kappaB signaling
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of cell migration
positive regulation of interferon-beta production
tumor necrosis factor-mediated signaling pathway
TRAIL-activated apoptotic signaling pathway
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of inflammatory response
positive regulation of multicellular organismal process
positive regulation of hair follicle development
cellular response to tumor necrosis factor
extrinsic apoptotic signaling pathway
NIK/NF-kappaB signaling
regulation of apoptotic process
apoptotic signaling pathway
positive regulation of execution phase of apoptosis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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