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Invitrogen
Cytokeratin 5/6 Polyclonal Antibody
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FIGURE: 1 / 6
Cytokeratin 5/6 Antibody (PA5-116450) in IHC (P)
Immunohistochemical analysis of Cytokeratin 5/6 in a paraffin-embedded human esophagus tissue using a Cytokeratin 5/6 polyclonal antibody (Product #PA5-116450). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-116450
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within Human Cytokeratin 5 aa 187-236.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive controls: A431 cell lysates, human skin tissue, human esophagus tissue, Hela.
Target Information
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 58 kDa cytokeratin; 60-kDa keratin; CK-5; CK-6B; cytokeratin 5; cytokeratin 6A; cytokeratin 6B; cytokeratin 6C; cytokeratin 6D; cytokeratin-5; cytokeratin-6A; cytokeratin-6B; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K6-alpha; K6B; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6A, , type II; keratin 6A, type II; keratin 6B, type II; keratin 8; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6b; keratin complex 2, basic, gene 6c; keratin K5; keratin type II; keratin, epidermal type II, K6A; keratin, epidermal, type II, K6B; keratin, type II cytoskeletal 6A; keratin-6-alpha; keratin-6-beta; keratin-like 1 (a type II keratin sequence); KRT5; mK6-alpha; mK6-beta; Sak57; tuftelin interacting protein 8; type-II keratin Kb10; type-II keratin Kb5; type-II keratin Kb6; unnamed protein product
Gene Aliases: 2310016L08Rik; 3300001P10Rik; CK-5; CK-6A; CK-6B; CK-6C; CK-6E; CK5; CK6A; CK6B; CK6C; CK6D; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; K5; K6[a]; K6A; K6B; K6C; K6D; Krt2-5; Krt2-6a; Krt2-6b; Krt2-6c; KRT5A; KRT6C; KRT6D; KRTL1; mK6[a]; mK6[b]; MK6a; PC2; PC3; PC4; Sak57; Tfip8
UniProt ID: (Human) P13647, (Human) P02538, (Human) P04259, (Mouse) Q922U2, (Rat) Q6P6Q2, (Mouse) P50446
Entrez Gene ID: (Human) 3852, (Human) 3853, (Human) 3854, (Mouse) 110308, (Rat) 369017, (Rat) 120093743, (Mouse) 16687, (Mouse) 16688
response to mechanical stimulus
regulation of cell migration
keratinization
regulation of protein localization
intermediate filament polymerization
intermediate filament organization
ectoderm development
negative regulation of cell migration
morphogenesis of an epithelium
positive regulation of cell proliferation
cell differentiation
killing of cells of other organism
wound healing
defense response to Gram-positive bacterium
antimicrobial humoral immune response mediated by antimicrobial peptide
negative regulation of entry of bacterium into host cell
Wnt signaling pathway
biological_process
epidermis development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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