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Invitrogen
CRF Polyclonal Antibody
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FIGURE: 1 / 1
CRF Antibody (PA5-116529) in WB
Western blot analysis of CRF in extracts of various cell lines. Samples were incubated with CRF Polyclonal antibody (Product # PA5-116529) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-116529
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.1 mg/mL
Amount
110 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: SH-SY5Y, Mouse liver, Mouse brain, Rat liver, Rat brain
Immunogen sequence: LLSRGPVPGA RQAPQHPQPL DFFQPPPQSE QPQQPQARPV LLRMGEEYFL RLGNLNKSPA APLSPASSLL AGGSGSRPSP EQATANFFRV LLQQLLLPRR SLDSPAALAE RGARNALGGH QEAPERERRS EEPPISLDLT FHLLREVLEM ARAEQLAQQA HSNRKLMEII
Target Information
Corticotropin Releasing Factor (CRF), a 41 amino acids neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is critical in the regulation of the pituitary-adrenal gland axis, and in endocrine, autonomic and behavioral responses to stress. CRF is widely distributed in the brain and peripheral nervous system with the highest levels in the hypothalamic paraventricular nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: corticoliberin; corticotropin releasing factor; corticotropin-releasing factor; unnamed protein product
Gene Aliases: CRF; CRH1; Gm1347
UniProt ID: (Human) P06850, (Mouse) Q8CIT0, (Rat) P01143
Entrez Gene ID: (Human) 1392, (Mouse) 12918, (Rat) 81648
positive regulation of protein phosphorylation
synaptic transmission, dopaminergic
negative regulation of systemic arterial blood pressure
glucocorticoid biosynthetic process
inflammatory response
neuropeptide signaling pathway
female pregnancy
learning or memory
memory
steroid metabolic process
positive regulation of cell proliferation
associative learning
hormone-mediated apoptotic signaling pathway
response to xenobiotic stimulus
positive regulation of gene expression
negative regulation of gene expression
negative regulation of norepinephrine secretion
regulation of serotonin secretion
diterpenoid metabolic process
hypothalamus development
lung development
adrenal gland development
regulation of synaptic transmission, GABAergic
negative regulation of epinephrine secretion
negative regulation of luteinizing hormone secretion
social behavior
locomotory exploration behavior
positive regulation of insulin secretion involved in cellular response to glucose stimulus
response to immobilization stress
response to cocaine
response to estrogen
response to ethanol
response to ether
response to pain
ion homeostasis
neuron apoptotic process
response to corticosterone
positive regulation of corticotropin secretion
positive regulation of cortisol secretion
general adaptation syndrome
long-term synaptic potentiation
positive regulation of digestive system process
gamma-aminobutyric acid secretion, neurotransmission
negative regulation of glucagon secretion
cellular response to cocaine
cellular response to dexamethasone stimulus
positive regulation of calcium ion import
response to alcohol
modulation of inhibitory postsynaptic potential
positive regulation of cAMP/PKA signal transduction
negative regulation of eating behavior
response to aldosterone
regulation of N-methyl-D-aspartate selective glutamate receptor activity
positive regulation of corticosterone secretion
positive regulation of behavioral fear response
signal transduction
chemical synaptic transmission
parturition
positive regulation of circadian sleep/wake cycle, wakefulness
negative regulation of circadian sleep/wake cycle, REM sleep
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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