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Invitrogen
GFI1 Polyclonal Antibody
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FIGURE: 1 / 1
GFI1 Antibody (PA5-116697) in WB
Western blot analysis of GFI1 in extracts of various cell lines. Samples were incubated with GFI1 Polyclonal antibody (Product # PA5-116697) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-116697
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GFI1 (NP_0011206871)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
3.02 mg/mL
Amount
302 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse spleen, Rat thymus, Rat spleen
Immunogen sequence: MPRSFLVKSK KAHSYHQPRS PGPDYSLRLE NVPAPSRADS TSNAGGAKAE PRDRLSPESQ LTEAPDRASA SPDSCEGSVC ERSSEFEDFW RPPSPSASPA
Target Information
GFI1 is a nuclear zinc finger (ZF) transcriptional repressor that plays an important role in hematopoiesis and inner ear development, and has been implicated in lymphomagenesis. The Gfi1 protein expression is regulated by the ubiquitin-proteasome system. It is reported that ubiquitin ligase Triad1 interacts with the DNA-binding domain of Gfi-1. Gfi-1 represses transcription by directly binding to the consensus DNA sequence in the promoters of its target genes. Deficiency of Gfi-1 causes neutropenia and an accumulation of granulomonocytic precursor cells that is reminiscent of a myelodysplastic syndrome in mice.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ94509; growth factor independent 1 transcription repressor; growth factor independent protein 1; Growth factor independent-1; unnamed protein product
Gene Aliases: Gfi-1; Pal-1; Pal1
UniProt ID: (Mouse) P70338, (Rat) Q07120
Entrez Gene ID: (Mouse) 14581, (Rat) 24388
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
enzyme-substrate adaptor activity
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
double-strand break repair via homologous recombination
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
mechanosensory behavior
negative regulation of cell fate specification
negative regulation of vitamin D biosynthetic process
negative regulation of neuron projection development
regulation of toll-like receptor signaling pathway
inner ear morphogenesis
auditory receptor cell differentiation
positive regulation of cell fate specification
cell fate commitment
negative regulation of transcription, DNA-templated
positive regulation of interleukin-6-mediated signaling pathway
cellular response to lipopolysaccharide
negative regulation of double-strand break repair via homologous recombination
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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