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Phospho-Aurora A/B/C (Thr288, Thr232, Thr198) Polyclonal Antibody

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Phospho-Aurora A/B/C (Thr288, Thr232, Thr198) Polyclonal Antibody

Product Details

PA5-117233

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around T288 of human AURKA/AURKB/AURKCAURKA (NP_0035912)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.33 mg/mL

Amount

233 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2901863

Product Specific Information

Positive Samples: HeLa

Immunogen sequence: RTTLC

Target Information

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AIM-1; ARK-3; aurora 1; aurora 2; aurora 3; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora- and Ipl1-like midbody-associated protein 1; aurora- and Ipl1-like midbody-associated protein 1 homolog; aurora-1; aurora-A; aurora-B; aurora-related kinase 1; aurora-related kinase 2; aurora-related kinase 3; aurora/IPL1-like kinase; aurora/IPL1-related kinase 1; aurora/IPL1-related kinase 2; aurora/IPL1-related kinase 3; aurora/IPL1/EG2 protein 2; breast tumor-amplified kinase; epididymis secretory protein Li 90; ipl1- and aurora-related kinase 1; protein kinase; protein kinase; chromosomal passenger protein; protein phosphatase 1, regulatory subunit 47; protein phosphatase 1, regulatory subunit 48; ratAurA; serine/threonine kinase 12; serine/threonine kinase 13 (aurora/IPL1-like); serine/threonine kinase 6; serine/threonine protein kinase 15; serine/threonine-protein kinase 12; serine/threonine-protein kinase 13; serine/threonine-protein kinase 15; serine/threonine-protein kinase 5; serine/threonine-protein kinase 6; serine/threonine-protein kinase aurora-A; serine/threonine-protein kinase aurora-B; serine/threonine-protein kinase aurora-C; serine/threonine-protein kinase Ayk1; unnamed protein product; zinc finger protein 264-like; zinc finger protein 805-like

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Gene Aliases: AIE2; AIK; AIK2; AIK3; AIM-1; AIM1; ARK-1; ARK-2; ARK1; ARK2; ARK3; AURA; AurB; AurC; aurkb-sv1; aurkb-sv2; aurora-C; BTAK; HEL-S-90; IPL1; PPP1R47; PPP1R48; SPGF5; STK-1; STK12; STK13; STK15; STK5; STK6; STK7

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UniProt ID: (Human) O14965, (Human) Q96GD4, (Human) Q9UQB9, (Rat) O55099

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Entrez Gene ID: (Human) 6790, (Human) 9212, (Human) 6795, (Rat) 261730, (Rat) 114592, (Rat) 292554

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding protein kinase binding protein heterodimerization activity protein serine kinase activity molecular function activator activity kinase binding histone H1-4S27 kinase activity ubiquitin protein ligase binding histone kinase activity (H3-S10 specific) histone kinase activity (H3-S28 specific) nucleotide binding
Process(es)
meiotic spindle organization microtubule cytoskeleton organization mitotic cell cycle chromatin remodeling protein phosphorylation spindle organization mitotic spindle organization spindle assembly involved in female meiosis I centrosome cycle mitotic centrosome separation response to wounding anterior/posterior axis specification negative regulation of gene expression peptidyl-serine phosphorylation regulation of protein stability response to estradiol positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of cytokinesis negative regulation of apoptotic process positive regulation of translation positive regulation of mitotic cell cycle meiotic cell cycle centrosome localization cilium disassembly protein localization to centrosome positive regulation of mitochondrial fission liver regeneration positive regulation of oocyte maturation positive regulation of meiotic cell cycle process involved in oocyte maturation neuron projection extension negative regulation of transcription from RNA polymerase II promoter negative regulation of B cell apoptotic process positive regulation of microtubule depolymerization positive regulation of telomere maintenance negative regulation of cytokinesis positive regulation of cytokinesis protein localization to kinetochore cellular response to UV cleavage furrow formation post-translational protein modification cell cycle G2/M phase transition mitotic cytokinesis checkpoint negative regulation of innate immune response mitotic spindle midzone assembly midbody abscission positive regulation of mitotic sister chromatid segregation negative regulation of protein localization to chromatin repair of mitotic kinetochore microtubule attachment defect negative regulation of cGAS/STING signaling pathway positive regulation of lateral attachment of mitotic spindle microtubules to kinetochore mitotic sister chromatid biorientation regulation of transcription, DNA-templated protein autophosphorylation mitotic cytokinesis regulation of microtubule-based process regulation of chromosome segregation positive regulation of cell cycle process positive regulation of mitotic cell cycle spindle assembly checkpoint mitotic spindle assembly regulation of signal transduction by p53 class mediator positive regulation of mitotic sister chromatid separation positive regulation of attachment of mitotic spindle microtubules to kinetochore positive regulation of mitotic cytokinesis G2/M transition of mitotic cell cycle regulation of G2/M transition of mitotic cell cycle negative regulation of protein binding positive regulation of mitotic nuclear division regulation of centrosome cycle attachment of spindle microtubules to kinetochore regulation of cell cycle process spindle assembly
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