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Invitrogen
Phospho-ERK1/2 (Thr202, Tyr204) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-ERK1/2 (Thr202, Tyr204) Antibody (PA5-117252) in WB
Western blot analysis of Phospho-ERK1/2 (Thr202, Tyr204) in extracts of Jurkat cells. Samples were incubated with Phospho-ERK1/2 (Thr202, Tyr204) Polyclonal antibody (Product # PA5-117252) using a dilution of 1:1,000 or ERK1/ERK2 antibody. Followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Jurkat cells were treated by PMA/TPA (200 nM) at 37°C for 10 minutes. Lysates/pro... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-117252
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_0027363)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.58 mg/mL
Amount
58 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Cellular location: caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Immunogen sequence: LTEYV
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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