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Invitrogen
Phospho-YAP1 (Ser127) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-YAP1 (Ser127) Antibody (PA5-117322) in WB
Western blot analysis of Phospho-YAP1 (Ser127) in Untreated (–) and treated (+) HeLa whole cell extracts (30 µg). Samples were separated with 10% SDS-PAGE and incubated in Phospho-YAP1 (Ser127) polyclonal antibody (Product # PA5-117322) using a dilution of 1:5,000.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-117322
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser127 of human YAP1. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.18 mg/mL
Amount
18 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Centrifuge briefly prior to opening.
Target Information
YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 65 kDa Yes-associated protein; protein yorkie homolog; transcriptional co-activator; unnamed protein product; Yes associated protein 1; yes-associated protein 2; yes-associated protein YAP65 homolog; yorkie homolog
Gene Aliases: COB1; YAP; YAP-1; YAP2; YAP65; YKI
UniProt ID: (Human) P46937
Entrez Gene ID: (Human) 10413
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
transcription corepressor activity
protein binding
proline-rich region binding
DNA-binding transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
tissue homeostasis
morphogenesis of an epithelium
heart process
cellular response to DNA damage stimulus
positive regulation of gene expression
negative regulation of gene expression
positive regulation of keratinocyte proliferation
positive regulation of cell growth
polarized epithelial cell differentiation
response to progesterone
organ growth
hippo signaling
negative regulation of hippo signaling
wound healing
negative regulation of fat cell differentiation
positive regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of growth
positive regulation of transcription from RNA polymerase II promoter
epithelial cell proliferation
positive regulation of epithelial cell proliferation
regulation of neurogenesis
positive regulation of cardiac muscle cell proliferation
intestinal epithelial cell development
cardiac muscle tissue regeneration
protein-containing complex assembly
interleukin-6-mediated signaling pathway
cellular response to gamma radiation
regulation of stem cell proliferation
positive regulation of protein localization to nucleus
negative regulation of cilium assembly
negative regulation of epithelial cell apoptotic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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