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Phospho-PI3K p85/p55 (Tyr467, Tyr199) Polyclonal Antibody

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Phospho-PI3K p85/p55 (Tyr467, Tyr199) Polyclonal Antibody

Product Details

PA5-118549

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PIK3R1(Accession P27986), corresponding to amino acid residues around phosphorylated Tyr199.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2903051

Product Specific Information

Antibody detects endogenous levels of PI3K p85/p55 only when phosphorylated at Tyr467(p85) or Tyr199(p55). The site Tyr467 was historically referenced as Tyr458.

Target Information

Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: growth factor receptor bound 1; OTTHUMP00000009783; OTTHUMP00000009784; p55PIK; p85-alpha; GRB1; phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55); phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase p55 subunit; phosphatidylinositol 3-kinase p85 alpha; phosphatidylinositol 3-kinase, regulartory subunit, polypeptide 2; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphatidylinositol 3-kinase-associated p-85 alpha; Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50); Phosphoinositide 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit beta; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphoinositide-3-kinase, regulatory subunit 2 (beta); phosphoinositide-3-kinase, regulatory subunit 3 (gamma); PI3 kinase-associated p85; PI3-kinase p85 subunit alpha; PI3-kinase p85 subunit beta; PI3-kinase p85 subunit gamma; PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit beta; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3-kinase subunit p85-beta; PI3K regulatory subunit alpha; PI3K regulatory subunit beta; PI3K regulatory subunit gamma; PI3K-p46; polypeptide 3; ptdIns-3-kinase p85-alpha; ptdIns-3-kinase p85-beta; ptdIns-3-kinase p85-gamma; ptdIns-3-kinase regulatory subunit alpha; ptdIns-3-kinase regulatory subunit beta; ptdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; PtdIns-3-kinase regulatory subunit p85-al; ptdIns-3-kinase regulatory subunit p85-alpha; ptdIns-3-kinase regulatory subunit p85-beta; RP4-533D7.2; unnamed protein product

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Gene Aliases: AGM7; GRB1; IMD36; MPPH; MPPH1; p50alpha; p55; p55-GAMMA; p55alpha; p55PIK; p85; p85-ALPHA; p85-BETA; p85alpha; P85B; p85beta; PI3K; PI3KA

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UniProt ID: (Human) P27986, (Human) O00459, (Human) Q92569, (Mouse) P26450, (Rat) Q63787, (Mouse) O08908, (Rat) Q63789, (Mouse) Q64143

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Entrez Gene ID: (Human) 5295, (Human) 5296, (Human) 8503, (Mouse) 18708, (Rat) 25513, (Mouse) 18709, (Rat) 29741, (Rat) 60664, (Mouse) 18710

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Function(s)
phosphotyrosine binding transmembrane receptor protein tyrosine kinase adaptor activity insulin receptor binding insulin-like growth factor receptor binding neurotrophin TRKA receptor binding protein binding kinase regulator activity kinase activator activity protein phosphatase binding phosphatidylinositol 3-kinase regulator activity phosphatidylinositol 3-kinase regulatory subunit binding ErbB-3 class receptor binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding 1-phosphatidylinositol-3-kinase regulator activity protein heterodimerization activity phosphatidylinositol kinase activity enzyme-substrate adaptor activity phosphatidylinositol 3-kinase activator activity receptor tyrosine kinase binding 1-phosphatidylinositol-3-kinase activity receptor binding platelet-derived growth factor receptor binding calmodulin binding protein kinase binding protein domain specific binding estrogen receptor binding ubiquitin protein ligase binding ATPase binding phosphoprotein binding
Process(es)
cell migration involved in sprouting angiogenesis insulin receptor signaling pathway positive regulation of gene expression protein kinase B signaling cellular glucose homeostasis positive regulation of protein phosphorylation negative regulation of cell-matrix adhesion glucose metabolic process protein phosphorylation protein import into nucleus negative regulation of cell adhesion signal transduction extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of heart rate positive regulation of lamellipodium assembly negative regulation of muscle cell apoptotic process cytokine-mediated signaling pathway negative regulation of cell-cell adhesion B cell differentiation positive regulation of cell migration positive regulation of tumor necrosis factor production response to insulin cellular response to insulin stimulus positive regulation of RNA splicing regulation of toll-like receptor 4 signaling pathway cellular response to UV response to endoplasmic reticulum stress interleukin-18-mediated signaling pathway phosphatidylinositol-3-phosphate biosynthetic process natural killer cell mediated cytotoxicity positive regulation of protein import into nucleus negative regulation of apoptotic process positive regulation of cellular component biogenesis positive regulation of myoblast differentiation negative regulation of osteoclast differentiation negative regulation of blood pressure negative regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter phosphatidylinositol metabolic process phosphatidylinositol phosphorylation insulin-like growth factor receptor signaling pathway positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation protein stabilization positive regulation of cellular component organization negative regulation of multicellular organismal process response to glucocorticoid positive regulation of filopodium assembly regulation of stress fiber assembly negative regulation of stress fiber assembly response to cAMP positive regulation of synapse assembly growth hormone receptor signaling pathway T follicular helper cell differentiation positive regulation of focal adhesion disassembly positive regulation of endoplasmic reticulum unfolded protein response regulation of protein localization to plasma membrane protein polyubiquitination immune response T cell differentiation substrate adhesion-dependent cell spreading positive regulation of glucose import positive regulation of protein localization to plasma membrane regulation of autophagy negative regulation of MAPK cascade regulation of actin filament polymerization positive regulation of cell adhesion negative regulation of cell migration involved in sprouting angiogenesis response to yeast response to nutrient response to xenobiotic stimulus response to fructose response to iron(II) ion response to estradiol response to progesterone response to testosterone response to amino acid response to ethanol response to fatty acid response to growth factor cellular response to fatty acid response to dexamethasone regulation of locomotion negative regulation of anoikis
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