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Invitrogen
CDK4 Polyclonal Antibody
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FIGURE: 1 / 9
CDK4 Antibody (PA5-119059) in IHC (P)
Immunohistochemistry (Paraffin) analysis of CDK4 in human breast cancer tissue using CDK4 Polyclonal Antibody (Product # PA5-119059) (10 µg/mL) and using PBS intead of the primary antibody as a control. The sample was then stained with HRP-Goat anti-Rabbit IgG polyclonal secondary antibody (2 µg/mL). DAB staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-119059
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein Cyclin Dependent Kinase 4. The antigen corresponds to amino acid range 6-295 of the target protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK4; cell division protein kinase 4; MGC14458; orf; PSK-J3; unnamed protein product
Gene Aliases: CMM3; MCPH31; PSK-J3
UniProt ID: (Human) P11802, (Rat) P35426
Entrez Gene ID: (Human) 1019, (Rat) 94201
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
signal transduction
positive regulation of cell proliferation
response to xenobiotic stimulus
regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of fibroblast proliferation
regulation of cell cycle
regulation of transcription initiation from RNA polymerase II promoter
lens development in camera-type eye
protein phosphorylation
circadian rhythm
response to toxic substance
organ regeneration
response to testosterone
regulation of cell proliferation
positive regulation of apoptotic process
positive regulation of translation
positive regulation of cell size
positive regulation of epithelial cell proliferation
response to hyperoxia
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to polyamine macromolecule
cellular response to phorbol 13-acetate 12-myristate
cellular response to ionomycin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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