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Invitrogen
P-Glycoprotein Polyclonal Antibody
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FIGURE: 1 / 2
P-Glycoprotein Antibody (PA5-120134) in WB
Western blot analysis of P-Glycoprotein in extracts of various cell lines. Samples were incubated with P-Glycoprotein Polyclonal antibody (Product # PA5-120134) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-120134
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 603-692 of human P Glycoprotein (NP_000918.2).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.19 mg/mL
Amount
119 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: Mouse liver, Mouse brain. The target is usually found in the following locations: Cell membrane, Multi-pass membrane protein.
Immunogen sequence: DGVIVEKGNH DELMKEKGIY FKLVTMQTAG NEVELENAAD ESKSEIDALE MSSNDSRSSL IRKRSTRRSV RGSQAQDRKL STKEALDESI
Target Information
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-binding cassette sub-family B member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B, member 1A; ecotropic viral integration site 32; mdr1a protein; multi-drug resistance 3; multidrug resistance protein 1; multidrug resistance protein 1B; multidrug resistance protein 3; multiple drug resistant 1a; P glycoprotein 1; P-glycoprotein 3; P-glycoprotein; mdr1a; phospholipid transporter ABCB1; unnamed protein product
Gene Aliases: Abcb1; Abcb4; Evi32; mdr; mdr-3; Mdr1; Mdr1a; Mdr1b; Mdr3; P-gp; Pgp; Pgy-1; Pgy-3; Pgy1; Pgy3
UniProt ID: (Mouse) P21447, (Mouse) P06795
Entrez Gene ID: (Mouse) 18671, (Mouse) 18669
ATP binding
xenobiotic-transporting ATPase activity
efflux transmembrane transporter activity
ATPase activity
transmembrane transporter activity
ubiquitin protein ligase binding
ATPase activity, coupled to transmembrane movement of substances
xenobiotic transporter activity
carboxylic acid transmembrane transporter activity
phosphatidylcholine-translocating ATPase activity
phosphatidylethanolamine flippase activity
ceramide floppase activity
ATPase-coupled intramembrane lipid transporter activity
floppase activity
phosphatidylethanolamine floppase activity
ABC-type transporter activity
G2/M transition of mitotic cell cycle
epidermal growth factor receptor signaling pathway
heart development
adult heart development
female gonad development
response to xenobiotic stimulus
response to toxic substance
gene expression
proteasomal protein catabolic process
neurogenesis
exploration behavior
aldosterone secretion
xenobiotic transport
phospholipid translocation
terpenoid transport
cardiac muscle cell differentiation
transmembrane transport
transepithelial transport
stem cell proliferation
neural tissue regeneration
ceramide translocation
export across plasma membrane
carboxylic acid transmembrane transport
drug transmembrane export
drug transport across blood-brain barrier
regulation of chloride transport
tissue homeostasis
protein acetylation
establishment of endothelial blood-brain barrier
neuron differentiation
Sertoli cell barrier remodeling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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