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PDK3 Polyclonal Antibody
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FIGURE: 1 / 1
PDK3 Antibody (PA5-120166) in WB
Western blot analysis of PDK3 in extracts of SH-SY5Y cells. Samples were incubated with PDK3 Polyclonal antibody (Product # PA5-120166) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-120166
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 137-406 of human PDK3 (NP_005382.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.6 mg/mL
Amount
60 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: SH-SY5Y. The target is usually found in the following locations: Mitochondrion matrix.
Immunogen sequence: FGFDPFISTN IQYFLDRFYT NRISFRMLIN QHTLLFGGDT NPVHPKHIGS IDPTCNVADV VKDAYETAKM LCEQYYLVAP ELEVEEFNAK APDKPIQVVY VPSHLFHMLF ELFKNSMRAT VELYEDRKEG YPAVKTLVTL GKEDLSIKIS DLGGGVPLRK IDRLFNYMYS TAPRPSLEPT RAAPLAGFGY GLPISRLYAR YFQGDLKLYS MEGVGTDAVI YLKALSSESF ERLPVFNKSA WRHYKTTPEA DDWSNPSSEP RDASKYKAKQ
Target Information
PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial; pyruvate dehydrogenase; pyruvate dehydrogenase kinase, isoenzyme 3; pyruvate dehydrogenase, lipoamide, kinase isozyme 3, mitochondrial; unnamed protein product
Gene Aliases: CMTX6; GS1-358P8.4
UniProt ID: (Human) Q15120
Entrez Gene ID: (Human) 5165
regulation of acetyl-CoA biosynthetic process from pyruvate
regulation of glucose metabolic process
peptidyl-serine phosphorylation
cellular response to stress
peroxisome proliferator activated receptor signaling pathway
cellular response to fatty acid
hypoxia-inducible factor-1alpha signaling pathway
regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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