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Invitrogen
HNF4A Polyclonal Antibody
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FIGURE: 1 / 1
HNF4A Antibody (PA5-120781) in WB
Western blot analysis of HNF4A in extracts of SW480 cells. Samples were incubated with HNF4A Polyclonal antibody (Product # PA5-120781) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-120781
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 226-325 of human HNF4A (NP_8491801)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.98 mg/mL
Amount
98 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: 293T, HT-29. The target is usually found in the following locations: Nucleus.
Immunogen sequence: EHLLLGATKR SMVFKDVLLL GNDYIVPRHC PELAEMSRVS IRILDELVLP FQELQIDDNE YAYLKAIIFF DPDAKGLSDP GKIKRLRSQV QVSLEDYIND
Target Information
Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is an orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ39654; hepatic nuclear factor 4 alpha; HNF4-alpha; HNF4a7; HNF4a8; HNF4a9; HNF4alpha10/11/12; nuclear receptor subfamily 2 group A member 1; transcription factor; transcription factor 14; transcription factor HNF-4
Gene Aliases: FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF-14; TCF14
UniProt ID: (Human) P41235
Entrez Gene ID: (Human) 3172
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
receptor binding
fatty acid binding
protein binding
zinc ion binding
protein homodimerization activity
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
xenobiotic metabolic process
blood coagulation
negative regulation of cell proliferation
response to glucose
regulation of lipid metabolic process
cell differentiation
negative regulation of cell growth
intracellular receptor signaling pathway
glucose homeostasis
cholesterol homeostasis
regulation of circadian rhythm
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of insulin secretion
lipid homeostasis
phospholipid homeostasis
regulation of growth hormone receptor signaling pathway
triglyceride homeostasis
positive regulation of ornithine catabolic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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