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Invitrogen
SPICE1 Polyclonal Antibody
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FIGURE: 1 / 1
SPICE1 Antibody (PA5-121147) in WB
Western blot analysis of SPICE1 in extracts of various cell lines. Samples were incubated with SPICE1 Polyclonal antibody (Product # PA5-121147) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-121147
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SPICE1 (NP_653319.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.78 mg/mL
Amount
178 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: 293T, HeLa, BT-474, MCF7. The target is usually found in the following locations: Cytoplasm, centriole, centrosome, cytoskeleton, microtubule organizing center, spindle.
Immunogen sequence: MSFVRVNRCG PRVGVRKTPK VKKKKTSVKQ EWDNTVTDLT VHRATPEDLV RRHEIHKSKN RALVHWELQE KALKRKWRKQ KPETLNLEKR RLSIMKEILS DQYQMQDVLE KSDHLIAAAK ELFPRRRTGF PNVTVAPDSS QGPIVVNQDP ITQSIFNESV IEPQALNDVD GEEEGTVNSQ SGESENENEL DNSLNSQSNT NTDRFLQQLT EENFELISKL
Target Information
Regulator required for centriole duplication, for proper bipolar spindle formation and chromosome congression in mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: coiled-coil domain containing 52; coiled-coil domain-containing protein 52; unnamed protein product
Gene Aliases: CCDC52; D16Ertd480e; RGD1310729; SPICE
UniProt ID: (Human) Q8N0Z3, (Rat) Q5RKG1, (Mouse) Q8C804
Entrez Gene ID: (Human) 152185, (Rat) 288111, (Mouse) 212514
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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