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          • Primary Antibodies ›
          • AKT1 Antibodies

          Invitrogen

          Phospho-AKT1 (Thr308) Polyclonal Antibody

          View all (196) AKT1 antibodies

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          Cite Phospho-AKT1 (Thr308) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          Phospho-AKT1 (Thr308) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-AKT1 (Thr308) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

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          Phospho-AKT1 (Thr308) Antibody (PA5-121271) in WB

          Western blot analysis of Phospho-AKT1 (Thr308) in extracts of Jurkat cells. Samples were incubated with Phospho-AKT1 (Thr308) Polyclonal antibody (Product # PA5-121271) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Jurkat cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. Lysates/proteins: 25 µg per lane. Blocking buffer... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-AKT1 (Thr308) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-AKT1 (Thr308) Polyclonal Antibody

          Product Details

          PA5-121271

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic phosphorylated peptide around T308 of human AKT1 (NP_005154.2).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.65 mg/mL

          Amount

          65 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2914843

          Product Specific Information

          Positive test controls include: Jurkat, NIH/3T3, C6. The target is usually found in the following locations: Cell membrane, Cytoplasm, Nucleus.

          Immunogen sequence: MKTFC

          Target Information

          AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: AKT; AKT1 kinase; AKT1M; Akt1m protein; C-AKT; CAKT; PKB; PKB ALPHA; PKB beta; PKB gamma; PKB-ALPHA; PKBG; PRKBA; PROTEIN KINASE B ALPHA; protein kinase B-alpha; PROTO-ONCOGENE C-AKT; proto-oncogene product; RAC; RAC-ALPHA; RAC-ALPHA SERINE/THREONINE-PROTEIN KINASE; RAC-PK-ALPHA; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; thymoma viral proto-oncogene 1; tumor rejection antigen peptide pRLib; unnamed protein product; V-AKT MURINE THYMOMA VIRAL ONCOGENE HOMOLOG 1; V-AKT MURINE THYMOMA VIRAL ONCOGENE-LIKE PROTEIN 1

          View more View less

          Gene Aliases: Akt; LTR-akt; PKB; PKB/Akt; PKBalpha; Rac

          View more View less

          UniProt ID: (Mouse) P31750

          View more View less

          Entrez Gene ID: (Mouse) 11651

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding calmodulin binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding kinase binding protein kinase binding nitric-oxide synthase regulator activity protein serine/threonine kinase inhibitor activity GTPase activating protein binding identical protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding potassium channel activator activity protein serine kinase activity protein serine/threonine kinase binding TORC2 complex binding
          Process(es)
          osteoblast differentiation maternal placenta development positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis cytoplasmic translation complement receptor mediated signaling pathway sphingosine-1-phosphate signaling pathway glycogen metabolic process glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process gluconeogenesis translation translational initiation protein phosphorylation ubiquitin-dependent protein catabolic process protein import into nucleus NLS-bearing protein import into nucleus ER to Golgi vesicle-mediated transport Golgi to plasma membrane transport activation-induced cell death of T cells inflammatory response cellular response to DNA damage stimulus response to oxidative stress cytoskeleton organization epidermal growth factor receptor signaling pathway smoothened signaling pathway germ cell development insulin receptor signaling pathway apoptotic mitochondrial changes cellular response to starvation response to heat response to hormone gene expression negative regulation of autophagy positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process positive regulation of mitochondrial membrane potential regulation of neuron projection development response to muscle activity phosphorylation cell migration protein ubiquitination peptidyl-serine phosphorylation peptidyl-threonine phosphorylation spinal cord development lateral motor column neuron differentiation oogenesis stage protein catabolic process positive regulation of cell growth regulation of cell migration positive regulation of cell migration negative regulation of cell migration anaphase-promoting complex-dependent catabolic process SCF-dependent proteasomal ubiquitin-dependent protein catabolic process negative regulation of protein ubiquitination regulation of myelination lipopolysaccharide-mediated signaling pathway response to nutrient levels cellular response to nutrient levels negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity response to food peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus regulation of protein localization negative regulation of superoxide anion generation positive regulation of peptidyl-serine phosphorylation negative regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor signaling pathway toll-like receptor 4 signaling pathway cellular response to amino acid starvation response to fluid shear stress cellular response to heat protein K29-linked ubiquitination intracellular signal transduction interleukin-18-mediated signaling pathway cellular response to vascular endothelial growth factor stimulus cellular response to platelet-derived growth factor stimulus cellular response to decreased oxygen levels SREBP-SCAP complex retention in endoplasmic reticulum tRNA stabilization calcium ion transmembrane import into mitochondrion PERK-mediated unfolded protein response TORC1 signaling positive regulation of protein import into nucleus glucose homeostasis regulation of apoptotic process Golgi to plasma membrane protein transport positive regulation of apoptotic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of cholesterol biosynthetic process positive regulation of fat cell differentiation positive regulation of cilium assembly positive regulation of glycogen biosynthetic process negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter negative regulation of translational initiation positive regulation of translational initiation positive regulation of glucose import negative regulation of JNK cascade positive regulation of organ growth protein autophosphorylation positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway behavioral response to pain positive regulation of smooth muscle cell proliferation protein stabilization striated muscle cell differentiation positive regulation of protein metabolic process protein maturation positive regulation of protein kinase B signaling response to growth hormone regulation of vesicle-mediated transport glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development protein localization to cilium response to UV-A protein localization to mitochondrion response to growth factor protein localization to endoplasmic reticulum protein K11-linked ubiquitination cellular response to mechanical stimulus cellular response to calcium ion cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to hypoxia cellular hyperosmotic salinity response tricarboxylic acid metabolic process maintenance of protein location in mitochondrion cellular response to sorbitol protein K6-linked ubiquitination negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis regulation of postsynapse organization tRNA (guanine-N7)-methylation regulation of tRNA methylation negative regulation of calcium import into the mitochondrion cellular response to oxidised low-density lipoprotein particle stimulus cGAS/STING signaling pathway negative regulation of protein localization to lysosome negative regulation of cGAS/STING signaling pathway positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to nucleus cellular response to peptide negative regulation of cilium assembly negative regulation of leukocyte cell-cell adhesion positive regulation of protein localization to plasma membrane negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway regulation of aerobic respiration negative regulation of PERK-mediated unfolded protein response negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of protein localization to endoplasmic reticulum positive regulation of anaphase-promoting complex-dependent catabolic process cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus positive regulation of protein localization to cell surface negative regulation of lymphocyte migration negative regulation of intrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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