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Invitrogen
Phospho-ULK1 (Ser757) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-ULK1 (Ser757) Antibody (PA5-121296) in WB
Western blot analysis of Phospho-ULK1 (Ser757) in extracts of A-431 cells. Samples were incubated with Phospho-ULK1 (Ser757) Polyclonal antibody (Product # PA5-121296) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. A-431 cells were treated by EGF (100 ng/mL) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins: 25 µg p... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-121296
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.13 mg/mL
Amount
213 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: A-431. The target is usually found in the following locations: Cytoplasm, Preautophagosomal structure, cytosol.
Target Information
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG1; ATG 1; ATG1 autophagy related 1 homolog; autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; ULK 1; UNC 51
Gene Aliases: ATG1; ATG1A; hATG1; UNC51; Unc51.1
UniProt ID: (Human) O75385
Entrez Gene ID: (Human) 8408
autophagosome assembly
mitophagy
protein phosphorylation
autophagy
signal transduction
axonogenesis
protein localization
negative regulation of cell proliferation
regulation of autophagy
positive regulation of autophagy
regulation of tumor necrosis factor-mediated signaling pathway
macroautophagy
regulation of macroautophagy
peptidyl-serine phosphorylation
neuron projection development
negative regulation of protein complex assembly
cellular response to nutrient levels
cellular response to stress
piecemeal microautophagy of nucleus
response to starvation
protein autophosphorylation
negative regulation of collateral sprouting
axon extension
reticulophagy
regulation of protein lipidation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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