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Invitrogen

Phospho-BAD (Ser26) Polyclonal Antibody

View all (57) BAD antibodies

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Product Details

PA5-143655

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-Bad (Ser-26) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 26 in mouse Bad. This peptide sequence is highly conserved in rat Bad, but is not found in human Bad.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Amount

10 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942883

Product Specific Information

The antibody detects a 23 kDa* doublet corresponding to the apparent molecular mass of phosphorylated Bad on SDS-PAGE immunoblots of mouse J774A.1 treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.

Target Information

The protein encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bcl-2-binding component 6; bcl-xL/Bcl-2-associated death promoter; bcl2 antagonist of cell death; OTTMUSP00000017561; OTTMUSP00000022400; unnamed protein product

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Gene Aliases: Bbc2

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UniProt ID: (Mouse) Q61337

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Entrez Gene ID: (Mouse) 12015

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Function(s)
protein binding phospholipid binding lipid binding protein kinase binding protein phosphatase binding protein phosphatase 2B binding protein kinase B binding 14-3-3 protein binding cysteine-type endopeptidase activator activity
Process(es)
release of cytochrome c from mitochondria glucose catabolic process apoptotic process cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage positive regulation of mitochondrial membrane potential cytokine-mediated signaling pathway positive regulation of insulin secretion negative regulation by symbiont of host apoptotic process positive regulation of insulin secretion involved in cellular response to glucose stimulus glucose homeostasis regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process type B pancreatic cell proliferation positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of proteolysis ADP metabolic process ATP metabolic process regulation of mitochondrial membrane permeability pore complex assembly positive regulation of epithelial cell proliferation symbiont-mediated activation of host apoptosis cellular response to nicotine cellular response to lipid positive regulation of release of cytochrome c from mitochondria apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand intrinsic apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway in response to osmotic stress positive regulation of granulosa cell apoptotic process positive regulation of type B pancreatic cell development
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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