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Invitrogen

Phospho-N-cadherin/E-cadherin (Tyr820, Tyr835, Tyr860) Polyclonal Antibody

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Product Details

PA5-143660

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-N-Cadherin (Tyr-860) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 860 in human N-cadherin. This sequence is conserved in human E-cadherin (Tyr-835), P-cadherin (Tyr-782), R-cadherin (Tyr-870), and has significant homology to VE-Cadherin (Tyr-733).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Amount

25 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942888

Product Specific Information

This antibody was cross-adsorbed to phospho-N-Cadherin (Tyr-820) and unphosphorylated N-cadherin (Tyr-860) peptides before affinity purification using phospho-N-cadherin (Tyr-860) peptide. In western blots, the antibody detects a 130 kDa* band corresponding to N-cadherin (Tyr-860) in human endothelial cells treated with pervanadate and detects a 120 kDa band corresponding to E-cadherin (Tyr-835) in human A431 cells treated with pervanadate.

Target Information

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to beta-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cadherin 2, type 1, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal; N-cadherin; N-cadherin 1; neural cadherin; neural cadherin precursor; unnamed protein product

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Gene Aliases: ACOGS; ADHD8; ARVD14; CD325; CDHN; CDw325; N-CAD; N-cadherin; NCAD

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UniProt ID: (Human) P19022, (Rat) Q9Z1Y3, (Mouse) P15116

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Entrez Gene ID: (Human) 1000, (Rat) 83501, (Mouse) 12558

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Function(s)
RNA binding calcium ion binding protein binding beta-catenin binding enzyme binding protein kinase binding protein phosphatase binding identical protein binding alpha-catenin binding gamma-catenin binding cadherin binding macromolecular complex binding nitric-oxide synthase binding protein tyrosine kinase binding
Process(es)
cell morphogenesis type B pancreatic cell development cell-cell junction assembly cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules synapse assembly glial cell differentiation neural crest cell development calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration telencephalon development cerebral cortex development regulation of myelination regulation of protein localization adherens junction organization regulation of Rho protein signal transduction positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin positive regulation of axon extension blood vessel morphogenesis brain morphogenesis homeostasis of number of cells regulation of axonogenesis modulation of synaptic transmission striated muscle cell differentiation radial glial cell differentiation neuroepithelial cell differentiation regulation of oligodendrocyte progenitor proliferation negative regulation of canonical Wnt signaling pathway mesenchymal cell migration synaptic vesicle clustering neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance cell-cell adhesion regulation of postsynaptic density protein 95 clustering positive regulation of cellular protein localization positive regulation of synaptic vesicle clustering detection of muscle stretch
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