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Invitrogen
Phospho-SPHK2 (Thr578) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-SPHK2 (Thr578) Antibody (PA5-143774) in WB
Western blot of Phospho-SPHK2 (Thr578) in human recombinant SK2 (lanes 1-4) and HeLa treated with calyculin A (lanes 5-8). The blots were untreated (lane 1, 3, 5 & 7) or treated with lambda phosphatase (lane 2, 4, 6 & 8), then probed with SPHK2 polyclonal antibody (Product # PA5-143773) (lanes 1, 2, 5 & 6) or Phospho-SPHK2 (Thr578) polyclonal antibody (Product # PA5-143774) (lanes 3, 4, 7, & 8).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-143774
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-SK2 (Thr-578) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Thr-578 in human SK2. This peptide sequence is highly conserved in rat and mouse SK2 proteins, and is not well conserved in SK1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects 70 kDa* proteins corresponding to the molecular mass of SK2 on SDS-PAGE immunoblots of human recombinant SK2 and endogenous SK2 in human HeLa cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
Target Information
SPHK2, along with SPHK1, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK2 is an important signaling molecule with intra- and extracellular functions and has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide raange of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: lipid kinase; SK2; SPHK2; unnamed protein product
Gene Aliases: SK 2; SK-2; Sk2; SPK 2; SPK-2; Spk2
UniProt ID: (Human) Q9NRA0, (Mouse) Q9JIA7
Entrez Gene ID: (Human) 56848, (Rat) 308589, (Mouse) 56632
blood vessel development
regulation of cytokine production
positive regulation of cytokine production involved in immune response
sphingosine-1-phosphate signaling pathway
sphinganine-1-phosphate biosynthetic process
sphingosine metabolic process
brain development
female pregnancy
positive regulation of cell proliferation
negative regulation of cell growth
positive regulation of interleukin-13 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of mast cell activation involved in immune response
intracellular signal transduction
regulation of apoptotic process
positive regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of mast cell degranulation
positive regulation of gene expression, epigenetic
sphingosine biosynthetic process
regulation of transport
regulation of reactive oxygen species biosynthetic process
cellular response to phorbol 13-acetate 12-myristate
regulation of cytochrome-c oxidase activity
positive regulation of ceramide biosynthetic process
regulation of ATP biosynthetic process
regulation of ceramide biosynthetic process
regulation of lipid metabolic process
sphingolipid biosynthetic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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